Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000400010 |
Resumo: | PURPOSE: To evaluate the effect of N-acetylcysteine, as a renoprotective agent, when administered early after anesthesia induction, against ischemia/reperfusion injury in rats anesthetized with isoflurane. METHODS: Eighteen male Wistar rats weighing > 300g were anesthetized with isoflurane. The internal jugular vein and the left carotid artery were dissected and cannulated. The animals were randomly divided into GAcetyl, receiving intravenous N-acetylcysteine, 300mg/kg, and GIsot, isotonic saline. After 30 minutes, right nephrectomy was performed and the left renal artery was clamped during 45 minutes. The animals were sacrificed after 48 hours and blood samples were taken after anesthetic induction and upon sacrificing of the animals to evaluate blood creatinine. The kidneys were sent for histological analysis. RESULTS: The variation in serum creatinine was 2.33mg/dL ± 2.21 in GAcetyl and 4.38mg/dL ± 2.13 in GIsot (p=0.074). Two animals presented intense tubular necrosis in GAcetyl, compared to 5 in GIsot. Only GAcetyl presented animals free of tubular necrosis (two) and tubular degeneration (one). CONCLUSION: After renal ischemia/reperfusion, the rats which were given N-acetylcysteine presented less variation in serum creatinine and milder kidney injuries than the control group. |
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Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isofluraneIsofluraneAcetylcysteineAcute Kidney InjuryRats, WistarPURPOSE: To evaluate the effect of N-acetylcysteine, as a renoprotective agent, when administered early after anesthesia induction, against ischemia/reperfusion injury in rats anesthetized with isoflurane. METHODS: Eighteen male Wistar rats weighing > 300g were anesthetized with isoflurane. The internal jugular vein and the left carotid artery were dissected and cannulated. The animals were randomly divided into GAcetyl, receiving intravenous N-acetylcysteine, 300mg/kg, and GIsot, isotonic saline. After 30 minutes, right nephrectomy was performed and the left renal artery was clamped during 45 minutes. The animals were sacrificed after 48 hours and blood samples were taken after anesthetic induction and upon sacrificing of the animals to evaluate blood creatinine. The kidneys were sent for histological analysis. RESULTS: The variation in serum creatinine was 2.33mg/dL ± 2.21 in GAcetyl and 4.38mg/dL ± 2.13 in GIsot (p=0.074). Two animals presented intense tubular necrosis in GAcetyl, compared to 5 in GIsot. Only GAcetyl presented animals free of tubular necrosis (two) and tubular degeneration (one). CONCLUSION: After renal ischemia/reperfusion, the rats which were given N-acetylcysteine presented less variation in serum creatinine and milder kidney injuries than the control group.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000400010Acta Cirúrgica Brasileira v.27 n.4 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000400010info:eu-repo/semantics/openAccessMansano,André MarquesVianna,Pedro Thadeu GalvãoFabris,Viciany EriqueSilva,Leopoldo Muniz daBraz,Leandro GobboCastiglia,Yara Marcondes Machadoeng2012-04-24T00:00:00Zoai:scielo:S0102-86502012000400010Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-04-24T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
title |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
spellingShingle |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane Mansano,André Marques Isoflurane Acetylcysteine Acute Kidney Injury Rats, Wistar |
title_short |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
title_full |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
title_fullStr |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
title_full_unstemmed |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
title_sort |
Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane |
author |
Mansano,André Marques |
author_facet |
Mansano,André Marques Vianna,Pedro Thadeu Galvão Fabris,Viciany Erique Silva,Leopoldo Muniz da Braz,Leandro Gobbo Castiglia,Yara Marcondes Machado |
author_role |
author |
author2 |
Vianna,Pedro Thadeu Galvão Fabris,Viciany Erique Silva,Leopoldo Muniz da Braz,Leandro Gobbo Castiglia,Yara Marcondes Machado |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mansano,André Marques Vianna,Pedro Thadeu Galvão Fabris,Viciany Erique Silva,Leopoldo Muniz da Braz,Leandro Gobbo Castiglia,Yara Marcondes Machado |
dc.subject.por.fl_str_mv |
Isoflurane Acetylcysteine Acute Kidney Injury Rats, Wistar |
topic |
Isoflurane Acetylcysteine Acute Kidney Injury Rats, Wistar |
description |
PURPOSE: To evaluate the effect of N-acetylcysteine, as a renoprotective agent, when administered early after anesthesia induction, against ischemia/reperfusion injury in rats anesthetized with isoflurane. METHODS: Eighteen male Wistar rats weighing > 300g were anesthetized with isoflurane. The internal jugular vein and the left carotid artery were dissected and cannulated. The animals were randomly divided into GAcetyl, receiving intravenous N-acetylcysteine, 300mg/kg, and GIsot, isotonic saline. After 30 minutes, right nephrectomy was performed and the left renal artery was clamped during 45 minutes. The animals were sacrificed after 48 hours and blood samples were taken after anesthetic induction and upon sacrificing of the animals to evaluate blood creatinine. The kidneys were sent for histological analysis. RESULTS: The variation in serum creatinine was 2.33mg/dL ± 2.21 in GAcetyl and 4.38mg/dL ± 2.13 in GIsot (p=0.074). Two animals presented intense tubular necrosis in GAcetyl, compared to 5 in GIsot. Only GAcetyl presented animals free of tubular necrosis (two) and tubular degeneration (one). CONCLUSION: After renal ischemia/reperfusion, the rats which were given N-acetylcysteine presented less variation in serum creatinine and milder kidney injuries than the control group. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.4 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126440957018112 |