Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000100011 |
Resumo: | PURPOSE: Liver ischemia-reperfusion injury is a phenomenon presents in events like liver resections and transplantation. The restoration of blood flow may leads to local and systemic injury. Several techniques have been developed in order to avoid or ameliorate ischemia-reperfusion injury in clinical situations. The application of a sttuter reperfusion after the ischemic event (postconditioning) could alters the hydrodynamics and stimulates endogenous mechanisms that attenuate the reperfusion injury. The present study was designed to evaluate the potential protective effect of postconditioning in a model of ischemia-reperfusion in rats. METHODS: Hepatic anterior pedicle of median and left anterolateral segments were exposed and clamped for 1 hour. Two hours later, clamp was released in two different ways: Control Group (n=7): clamp was release straightforward; Postconditioning Group (n=7): clamp was released intermittently. Lipid peroxidation (malondialdehyde) and expression of the glutathione-s-transferase-α-3 gene were studied. RESULTS: Lipid peroxidation was significantly decreased in ischemic and non-ischemic liver by postconditioning. GST- α3 gene was overexpressed in postconditioned group, but not significantly. CONCLUSION: Postconditioning induced hepatoprotection by reducing lipid peroxidation in the ischemic and non-ischemic liver. |
id |
SBDPC-1_3a887ba6f359adb93913f8ee62c70440 |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502009000100011 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in ratsLiverIschemiaReperfusionLipid PeroxidationRatsPURPOSE: Liver ischemia-reperfusion injury is a phenomenon presents in events like liver resections and transplantation. The restoration of blood flow may leads to local and systemic injury. Several techniques have been developed in order to avoid or ameliorate ischemia-reperfusion injury in clinical situations. The application of a sttuter reperfusion after the ischemic event (postconditioning) could alters the hydrodynamics and stimulates endogenous mechanisms that attenuate the reperfusion injury. The present study was designed to evaluate the potential protective effect of postconditioning in a model of ischemia-reperfusion in rats. METHODS: Hepatic anterior pedicle of median and left anterolateral segments were exposed and clamped for 1 hour. Two hours later, clamp was released in two different ways: Control Group (n=7): clamp was release straightforward; Postconditioning Group (n=7): clamp was released intermittently. Lipid peroxidation (malondialdehyde) and expression of the glutathione-s-transferase-α-3 gene were studied. RESULTS: Lipid peroxidation was significantly decreased in ischemic and non-ischemic liver by postconditioning. GST- α3 gene was overexpressed in postconditioned group, but not significantly. CONCLUSION: Postconditioning induced hepatoprotection by reducing lipid peroxidation in the ischemic and non-ischemic liver.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2009-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000100011Acta Cirúrgica Brasileira v.24 n.1 2009reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502009000100011info:eu-repo/semantics/openAccessTeixeira,Antonio Roberto FranchiMolan,Nilza T.Kubrusly,Márcia SaldanhaBellodi-Privato,MartaCoelho,Ana MariaLeite,Kátia R.Machado,Marcel Autran CesarBacchella,TelésforoMachado,Marcel Cerqueira Césareng2009-01-14T00:00:00Zoai:scielo:S0102-86502009000100011Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2009-01-14T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
title |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
spellingShingle |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats Teixeira,Antonio Roberto Franchi Liver Ischemia Reperfusion Lipid Peroxidation Rats |
title_short |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
title_full |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
title_fullStr |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
title_full_unstemmed |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
title_sort |
Postconditioning ameliorates lipid peroxidation in liver ischemia-reperfusion injury in rats |
author |
Teixeira,Antonio Roberto Franchi |
author_facet |
Teixeira,Antonio Roberto Franchi Molan,Nilza T. Kubrusly,Márcia Saldanha Bellodi-Privato,Marta Coelho,Ana Maria Leite,Kátia R. Machado,Marcel Autran Cesar Bacchella,Telésforo Machado,Marcel Cerqueira César |
author_role |
author |
author2 |
Molan,Nilza T. Kubrusly,Márcia Saldanha Bellodi-Privato,Marta Coelho,Ana Maria Leite,Kátia R. Machado,Marcel Autran Cesar Bacchella,Telésforo Machado,Marcel Cerqueira César |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Teixeira,Antonio Roberto Franchi Molan,Nilza T. Kubrusly,Márcia Saldanha Bellodi-Privato,Marta Coelho,Ana Maria Leite,Kátia R. Machado,Marcel Autran Cesar Bacchella,Telésforo Machado,Marcel Cerqueira César |
dc.subject.por.fl_str_mv |
Liver Ischemia Reperfusion Lipid Peroxidation Rats |
topic |
Liver Ischemia Reperfusion Lipid Peroxidation Rats |
description |
PURPOSE: Liver ischemia-reperfusion injury is a phenomenon presents in events like liver resections and transplantation. The restoration of blood flow may leads to local and systemic injury. Several techniques have been developed in order to avoid or ameliorate ischemia-reperfusion injury in clinical situations. The application of a sttuter reperfusion after the ischemic event (postconditioning) could alters the hydrodynamics and stimulates endogenous mechanisms that attenuate the reperfusion injury. The present study was designed to evaluate the potential protective effect of postconditioning in a model of ischemia-reperfusion in rats. METHODS: Hepatic anterior pedicle of median and left anterolateral segments were exposed and clamped for 1 hour. Two hours later, clamp was released in two different ways: Control Group (n=7): clamp was release straightforward; Postconditioning Group (n=7): clamp was released intermittently. Lipid peroxidation (malondialdehyde) and expression of the glutathione-s-transferase-α-3 gene were studied. RESULTS: Lipid peroxidation was significantly decreased in ischemic and non-ischemic liver by postconditioning. GST- α3 gene was overexpressed in postconditioned group, but not significantly. CONCLUSION: Postconditioning induced hepatoprotection by reducing lipid peroxidation in the ischemic and non-ischemic liver. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502009000100011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.24 n.1 2009 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439515226112 |