Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats

• Autor(a) principal: Burlamaqui,Idália Maria Brasil
• Data de Publicação: 2010
• Outros Autores: Dornelas,Conceição Aparecida, Escalante,Rodrigo Dornenfeld, Mota,Daniel Magalhães Coutinho, Mesquita,Francisco José Cabral, Carvalho,Eduardo Rebouças, Veras,Lara Burlamaqui, Rodrigues,Lusmar Veras
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005
• topic: Precancerous Conditions; Colorectal Neoplasms; Azoxymethane; Rats

PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.