Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005 |
Resumo: | PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde. |
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Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar ratsPrecancerous ConditionsColorectal NeoplasmsAzoxymethaneRatsPURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005Acta Cirúrgica Brasileira v.25 n.2 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000200005info:eu-repo/semantics/openAccessBurlamaqui,Idália Maria BrasilDornelas,Conceição AparecidaEscalante,Rodrigo DornenfeldMota,Daniel Magalhães CoutinhoMesquita,Francisco José CabralCarvalho,Eduardo RebouçasVeras,Lara BurlamaquiRodrigues,Lusmar Veraseng2010-10-18T00:00:00Zoai:scielo:S0102-86502010000200005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-10-18T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
title |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
spellingShingle |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats Burlamaqui,Idália Maria Brasil Precancerous Conditions Colorectal Neoplasms Azoxymethane Rats |
title_short |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
title_full |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
title_fullStr |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
title_full_unstemmed |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
title_sort |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats |
author |
Burlamaqui,Idália Maria Brasil |
author_facet |
Burlamaqui,Idália Maria Brasil Dornelas,Conceição Aparecida Escalante,Rodrigo Dornenfeld Mota,Daniel Magalhães Coutinho Mesquita,Francisco José Cabral Carvalho,Eduardo Rebouças Veras,Lara Burlamaqui Rodrigues,Lusmar Veras |
author_role |
author |
author2 |
Dornelas,Conceição Aparecida Escalante,Rodrigo Dornenfeld Mota,Daniel Magalhães Coutinho Mesquita,Francisco José Cabral Carvalho,Eduardo Rebouças Veras,Lara Burlamaqui Rodrigues,Lusmar Veras |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Burlamaqui,Idália Maria Brasil Dornelas,Conceição Aparecida Escalante,Rodrigo Dornenfeld Mota,Daniel Magalhães Coutinho Mesquita,Francisco José Cabral Carvalho,Eduardo Rebouças Veras,Lara Burlamaqui Rodrigues,Lusmar Veras |
dc.subject.por.fl_str_mv |
Precancerous Conditions Colorectal Neoplasms Azoxymethane Rats |
topic |
Precancerous Conditions Colorectal Neoplasms Azoxymethane Rats |
description |
PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502010000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.25 n.2 2010 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439946190848 |