Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000100010 |
Resumo: | PURPOSE: To compare the viability of human hepatocytes dissociated by the ethylenediaminetetraacetic acid and collagenase techniques. METHODS: Hepatocytes were prepared by dissociation of liver fragments obtained from hepatectomies performed for therapeutic purposes at the Service of Digestive Tract Surgery, Federal University of Triângulo Mineiro. RESULTS: During the first 4 days of the experiment, 70% of the cells presented birefringent membranes and were not stained with 2% erythrosine, and were therefore considered to be viable. During the first 3 days, hepatocyte viability was on average 71% in the EDTA group and 76% in the collagenase group, with no significant difference between groups. No significant difference was observed between groups at any time. The secretion of albumin by the cultured hepatocytes was preserved up to the seventh day. Mean albumin secretion during the first 3 days was 50 µg/ml in the two groups and a reduction of albumin production was observed from the fourth to the seventh day. Again, no significant difference was observed between groups at any time. CONCLUSION: Cell viability and preservation of albumin secretion by hepatocytes are similar for the EDTA and collagenase techniques. |
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Acta Cirúrgica Brasileira (Online) |
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Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase techniqueCell SeparationCell TransplantationEdetic AcidPURPOSE: To compare the viability of human hepatocytes dissociated by the ethylenediaminetetraacetic acid and collagenase techniques. METHODS: Hepatocytes were prepared by dissociation of liver fragments obtained from hepatectomies performed for therapeutic purposes at the Service of Digestive Tract Surgery, Federal University of Triângulo Mineiro. RESULTS: During the first 4 days of the experiment, 70% of the cells presented birefringent membranes and were not stained with 2% erythrosine, and were therefore considered to be viable. During the first 3 days, hepatocyte viability was on average 71% in the EDTA group and 76% in the collagenase group, with no significant difference between groups. No significant difference was observed between groups at any time. The secretion of albumin by the cultured hepatocytes was preserved up to the seventh day. Mean albumin secretion during the first 3 days was 50 µg/ml in the two groups and a reduction of albumin production was observed from the fourth to the seventh day. Again, no significant difference was observed between groups at any time. CONCLUSION: Cell viability and preservation of albumin secretion by hepatocytes are similar for the EDTA and collagenase techniques.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2007-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000100010Acta Cirúrgica Brasileira v.22 n.1 2007reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502007000100010info:eu-repo/semantics/openAccessCrema,EduardoWerneck,Ana MárciaMartins Júnior,AiodairRibeiro,Lara Beatriz PrataLima,Tatiana Silva deSilva,Alex Augustoeng2007-03-15T00:00:00Zoai:scielo:S0102-86502007000100010Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2007-03-15T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
title |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
spellingShingle |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique Crema,Eduardo Cell Separation Cell Transplantation Edetic Acid |
title_short |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
title_full |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
title_fullStr |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
title_full_unstemmed |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
title_sort |
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique |
author |
Crema,Eduardo |
author_facet |
Crema,Eduardo Werneck,Ana Márcia Martins Júnior,Aiodair Ribeiro,Lara Beatriz Prata Lima,Tatiana Silva de Silva,Alex Augusto |
author_role |
author |
author2 |
Werneck,Ana Márcia Martins Júnior,Aiodair Ribeiro,Lara Beatriz Prata Lima,Tatiana Silva de Silva,Alex Augusto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Crema,Eduardo Werneck,Ana Márcia Martins Júnior,Aiodair Ribeiro,Lara Beatriz Prata Lima,Tatiana Silva de Silva,Alex Augusto |
dc.subject.por.fl_str_mv |
Cell Separation Cell Transplantation Edetic Acid |
topic |
Cell Separation Cell Transplantation Edetic Acid |
description |
PURPOSE: To compare the viability of human hepatocytes dissociated by the ethylenediaminetetraacetic acid and collagenase techniques. METHODS: Hepatocytes were prepared by dissociation of liver fragments obtained from hepatectomies performed for therapeutic purposes at the Service of Digestive Tract Surgery, Federal University of Triângulo Mineiro. RESULTS: During the first 4 days of the experiment, 70% of the cells presented birefringent membranes and were not stained with 2% erythrosine, and were therefore considered to be viable. During the first 3 days, hepatocyte viability was on average 71% in the EDTA group and 76% in the collagenase group, with no significant difference between groups. No significant difference was observed between groups at any time. The secretion of albumin by the cultured hepatocytes was preserved up to the seventh day. Mean albumin secretion during the first 3 days was 50 µg/ml in the two groups and a reduction of albumin production was observed from the fourth to the seventh day. Again, no significant difference was observed between groups at any time. CONCLUSION: Cell viability and preservation of albumin secretion by hepatocytes are similar for the EDTA and collagenase techniques. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000100010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502007000100010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.22 n.1 2007 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126438602964992 |