Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury
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Publication Date: | 2012 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Acta Cirúrgica Brasileira (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001100006 |
Summary: | PURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress. |
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Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injuryOxidative StressAntioxidantsGene expressionIschemiaReperfusionReal-Time Polymerase Chain ReactionMicePURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001100006Acta Cirúrgica Brasileira v.27 n.11 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012001100006info:eu-repo/semantics/openAccessBertoletto,Paulo RobertoIkejiri,Adauto TsutomuSomaio Neto,FredericoChaves,José CarlosTeruya,RobertoBertoletto,Eduardo RodriguesTaha,Murched OmarFagundes,Djalma Joséeng2012-10-29T00:00:00Zoai:scielo:S0102-86502012001100006Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-10-29T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
title |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
spellingShingle |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury Bertoletto,Paulo Roberto Oxidative Stress Antioxidants Gene expression Ischemia Reperfusion Real-Time Polymerase Chain Reaction Mice |
title_short |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
title_full |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
title_fullStr |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
title_full_unstemmed |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
title_sort |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury |
author |
Bertoletto,Paulo Roberto |
author_facet |
Bertoletto,Paulo Roberto Ikejiri,Adauto Tsutomu Somaio Neto,Frederico Chaves,José Carlos Teruya,Roberto Bertoletto,Eduardo Rodrigues Taha,Murched Omar Fagundes,Djalma José |
author_role |
author |
author2 |
Ikejiri,Adauto Tsutomu Somaio Neto,Frederico Chaves,José Carlos Teruya,Roberto Bertoletto,Eduardo Rodrigues Taha,Murched Omar Fagundes,Djalma José |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bertoletto,Paulo Roberto Ikejiri,Adauto Tsutomu Somaio Neto,Frederico Chaves,José Carlos Teruya,Roberto Bertoletto,Eduardo Rodrigues Taha,Murched Omar Fagundes,Djalma José |
dc.subject.por.fl_str_mv |
Oxidative Stress Antioxidants Gene expression Ischemia Reperfusion Real-Time Polymerase Chain Reaction Mice |
topic |
Oxidative Stress Antioxidants Gene expression Ischemia Reperfusion Real-Time Polymerase Chain Reaction Mice |
description |
PURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001100006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012001100006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.11 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441364914176 |