The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43

Detalhes bibliográficos
Autor(a) principal: Trevisan,Alexandre Maximiliano
Data de Publicação: 2019
Outros Autores: Cogliati,Bruno, Homem,Adriana Ribeiro, Aloiav,Thiago Pinheiro Arrais, Aquino Neto,Nelson de, Moreira,Jairo Marques, Reno,Leonardo da Cruz, Naumann,Alexandre Moulin, Galvão,Flavio Henrique Ferreira, Andraus,Wellington, D'Albuquerque,Luiz Augusto Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001000212
Resumo: Abstract Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.
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spelling The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43GAP junctionsConnexin 43IschemiaReperfusionLiverCell communicationMiceAbstract Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2019-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001000212Acta Cirúrgica Brasileira v.34 n.10 2019reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020190100000003info:eu-repo/semantics/openAccessTrevisan,Alexandre MaximilianoCogliati,BrunoHomem,Adriana RibeiroAloiav,Thiago Pinheiro ArraisAquino Neto,Nelson deMoreira,Jairo MarquesReno,Leonardo da CruzNaumann,Alexandre MoulinGalvão,Flavio Henrique FerreiraAndraus,WellingtonD'Albuquerque,Luiz Augusto Carneiroeng2019-12-10T00:00:00Zoai:scielo:S0102-86502019001000212Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2019-12-10T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
title The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
spellingShingle The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
Trevisan,Alexandre Maximiliano
GAP junctions
Connexin 43
Ischemia
Reperfusion
Liver
Cell communication
Mice
title_short The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
title_full The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
title_fullStr The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
title_full_unstemmed The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
title_sort The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43
author Trevisan,Alexandre Maximiliano
author_facet Trevisan,Alexandre Maximiliano
Cogliati,Bruno
Homem,Adriana Ribeiro
Aloiav,Thiago Pinheiro Arrais
Aquino Neto,Nelson de
Moreira,Jairo Marques
Reno,Leonardo da Cruz
Naumann,Alexandre Moulin
Galvão,Flavio Henrique Ferreira
Andraus,Wellington
D'Albuquerque,Luiz Augusto Carneiro
author_role author
author2 Cogliati,Bruno
Homem,Adriana Ribeiro
Aloiav,Thiago Pinheiro Arrais
Aquino Neto,Nelson de
Moreira,Jairo Marques
Reno,Leonardo da Cruz
Naumann,Alexandre Moulin
Galvão,Flavio Henrique Ferreira
Andraus,Wellington
D'Albuquerque,Luiz Augusto Carneiro
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trevisan,Alexandre Maximiliano
Cogliati,Bruno
Homem,Adriana Ribeiro
Aloiav,Thiago Pinheiro Arrais
Aquino Neto,Nelson de
Moreira,Jairo Marques
Reno,Leonardo da Cruz
Naumann,Alexandre Moulin
Galvão,Flavio Henrique Ferreira
Andraus,Wellington
D'Albuquerque,Luiz Augusto Carneiro
dc.subject.por.fl_str_mv GAP junctions
Connexin 43
Ischemia
Reperfusion
Liver
Cell communication
Mice
topic GAP junctions
Connexin 43
Ischemia
Reperfusion
Liver
Cell communication
Mice
description Abstract Purpose: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. Methods: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. Results: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. Conclusions: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001000212
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001000212
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020190100000003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.34 n.10 2019
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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