Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?

• Autor(a) principal: Çakır,Tuğrul
• Data de Publicação: 2015
• Outros Autores: Baştürk,Ahmet, Polat,Cemal, Aslaner,Arif, Durgut,Himmet, Şehirli,Ahmet Özer, Gül,Mehmet, Öğünç,Ayliz Velioğlu, Gül,Semir, Sabuncuoglu,Mehmet Zafer, Oruç,Mehmet Tahir
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000400247
• topic: Thioctic Acid; Methotrexate; Liver; Rats

PURPOSE: To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.METHODS:Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.RESULTS: MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL- β) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.CONCLUSION:Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects.