Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats

Detalhes bibliográficos
Autor(a) principal: Leite,Camila Ferreira
Data de Publicação: 2013
Outros Autores: Marangoni,Fábio André, Camargo,Enilton Aparecido, Braga,Angélica de Fátima de Assunção, Toro,Ivan Felizardo Contrera, Antunes,Edson, Landucci,Elen Cristina Tiezem, Mussi,Ricardo Kalaf
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000400003
Resumo: PURPOSE: To investigate the anti-inflammatory effects of simvastatin in rats undergoing one-lung ventilation (OLV) followed by lung re-expansion. METHODS: Male Wistar rats (n=30) were submitted to 1-h OLV followed by 1-h lung re-expansion. Treated group received simvastatin (40 mg/kg for 21 days) previous to OLV protocol. Control group received no treatment or surgical/ventilation interventions. Measurements of pulmonary myeloperoxidase (MPO) activity, pulmonary protein extravasation, and serum levels of cytokines and C-reactive protein (CRP) were performed. RESULTS: OLV significantly increased the MPO activity in the collapsed and continuously ventilated lungs (31% and 52% increase, respectively) compared with control (p<0.05). Treatment with simvastatin significantly reduced the MPO activity in the continuously ventilated lung but had no effect on lung edema after OLV. The serum IL-6 and CRP levels were markedly higher in OLV group, but simvastatin treatment failed to affect the production of these inflammatory markers. Serum levels of IL-1β, TNF-α and IL-10 remained below the detection limit in all groups. CONCLUSIONS: In an experimental one-lung ventilation model pre-operative treatment with simvastatin reduces remote neutrophil infiltration in the continuously ventilated lung. Our findings suggest that simvastatin may be of therapeutic value in OLV-induced pulmonary inflammation deserving clinical investigations.
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spelling Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in ratsModels, AnimalPulmonary VentilationSimvastatinThoracic SurgeryInflammationRatsPURPOSE: To investigate the anti-inflammatory effects of simvastatin in rats undergoing one-lung ventilation (OLV) followed by lung re-expansion. METHODS: Male Wistar rats (n=30) were submitted to 1-h OLV followed by 1-h lung re-expansion. Treated group received simvastatin (40 mg/kg for 21 days) previous to OLV protocol. Control group received no treatment or surgical/ventilation interventions. Measurements of pulmonary myeloperoxidase (MPO) activity, pulmonary protein extravasation, and serum levels of cytokines and C-reactive protein (CRP) were performed. RESULTS: OLV significantly increased the MPO activity in the collapsed and continuously ventilated lungs (31% and 52% increase, respectively) compared with control (p<0.05). Treatment with simvastatin significantly reduced the MPO activity in the continuously ventilated lung but had no effect on lung edema after OLV. The serum IL-6 and CRP levels were markedly higher in OLV group, but simvastatin treatment failed to affect the production of these inflammatory markers. Serum levels of IL-1β, TNF-α and IL-10 remained below the detection limit in all groups. CONCLUSIONS: In an experimental one-lung ventilation model pre-operative treatment with simvastatin reduces remote neutrophil infiltration in the continuously ventilated lung. Our findings suggest that simvastatin may be of therapeutic value in OLV-induced pulmonary inflammation deserving clinical investigations.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000400003Acta Cirúrgica Brasileira v.28 n.4 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013000400003info:eu-repo/semantics/openAccessLeite,Camila FerreiraMarangoni,Fábio AndréCamargo,Enilton AparecidoBraga,Angélica de Fátima de AssunçãoToro,Ivan Felizardo ContreraAntunes,EdsonLanducci,Elen Cristina TiezemMussi,Ricardo Kalafeng2013-04-02T00:00:00Zoai:scielo:S0102-86502013000400003Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-04-02T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
title Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
spellingShingle Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
Leite,Camila Ferreira
Models, Animal
Pulmonary Ventilation
Simvastatin
Thoracic Surgery
Inflammation
Rats
title_short Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
title_full Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
title_fullStr Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
title_full_unstemmed Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
title_sort Simvastatin attenuates neutrophil recruitment in one-lung ventilation model in rats
author Leite,Camila Ferreira
author_facet Leite,Camila Ferreira
Marangoni,Fábio André
Camargo,Enilton Aparecido
Braga,Angélica de Fátima de Assunção
Toro,Ivan Felizardo Contrera
Antunes,Edson
Landucci,Elen Cristina Tiezem
Mussi,Ricardo Kalaf
author_role author
author2 Marangoni,Fábio André
Camargo,Enilton Aparecido
Braga,Angélica de Fátima de Assunção
Toro,Ivan Felizardo Contrera
Antunes,Edson
Landucci,Elen Cristina Tiezem
Mussi,Ricardo Kalaf
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leite,Camila Ferreira
Marangoni,Fábio André
Camargo,Enilton Aparecido
Braga,Angélica de Fátima de Assunção
Toro,Ivan Felizardo Contrera
Antunes,Edson
Landucci,Elen Cristina Tiezem
Mussi,Ricardo Kalaf
dc.subject.por.fl_str_mv Models, Animal
Pulmonary Ventilation
Simvastatin
Thoracic Surgery
Inflammation
Rats
topic Models, Animal
Pulmonary Ventilation
Simvastatin
Thoracic Surgery
Inflammation
Rats
description PURPOSE: To investigate the anti-inflammatory effects of simvastatin in rats undergoing one-lung ventilation (OLV) followed by lung re-expansion. METHODS: Male Wistar rats (n=30) were submitted to 1-h OLV followed by 1-h lung re-expansion. Treated group received simvastatin (40 mg/kg for 21 days) previous to OLV protocol. Control group received no treatment or surgical/ventilation interventions. Measurements of pulmonary myeloperoxidase (MPO) activity, pulmonary protein extravasation, and serum levels of cytokines and C-reactive protein (CRP) were performed. RESULTS: OLV significantly increased the MPO activity in the collapsed and continuously ventilated lungs (31% and 52% increase, respectively) compared with control (p<0.05). Treatment with simvastatin significantly reduced the MPO activity in the continuously ventilated lung but had no effect on lung edema after OLV. The serum IL-6 and CRP levels were markedly higher in OLV group, but simvastatin treatment failed to affect the production of these inflammatory markers. Serum levels of IL-1β, TNF-α and IL-10 remained below the detection limit in all groups. CONCLUSIONS: In an experimental one-lung ventilation model pre-operative treatment with simvastatin reduces remote neutrophil infiltration in the continuously ventilated lung. Our findings suggest that simvastatin may be of therapeutic value in OLV-induced pulmonary inflammation deserving clinical investigations.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502013000400003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.28 n.4 2013
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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