Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep

Detalhes bibliográficos
Autor(a) principal: Brandão,Aline Grama Moreira
Data de Publicação: 2018
Outros Autores: Consalter,Angélica, Leite,Juliana da Silva, Zamprogno,Helia, Ferreira,Ana Maria Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231
Resumo: Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option.
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spelling Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheepMusclesRegenerationStem CellsModels, AnimalSheep.Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231Acta Cirúrgica Brasileira v.33 n.3 2018reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020180030000005info:eu-repo/semantics/openAccessBrandão,Aline Grama MoreiraConsalter,AngélicaLeite,Juliana da SilvaZamprogno,HeliaFerreira,Ana Maria Reiseng2018-04-09T00:00:00Zoai:scielo:S0102-86502018000300231Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2018-04-09T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
title Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
spellingShingle Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
Brandão,Aline Grama Moreira
Muscles
Regeneration
Stem Cells
Models, Animal
Sheep.
title_short Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
title_full Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
title_fullStr Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
title_full_unstemmed Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
title_sort Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
author Brandão,Aline Grama Moreira
author_facet Brandão,Aline Grama Moreira
Consalter,Angélica
Leite,Juliana da Silva
Zamprogno,Helia
Ferreira,Ana Maria Reis
author_role author
author2 Consalter,Angélica
Leite,Juliana da Silva
Zamprogno,Helia
Ferreira,Ana Maria Reis
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Brandão,Aline Grama Moreira
Consalter,Angélica
Leite,Juliana da Silva
Zamprogno,Helia
Ferreira,Ana Maria Reis
dc.subject.por.fl_str_mv Muscles
Regeneration
Stem Cells
Models, Animal
Sheep.
topic Muscles
Regeneration
Stem Cells
Models, Animal
Sheep.
description Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020180030000005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.33 n.3 2018
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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