Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231 |
Resumo: | Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option. |
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Acta Cirúrgica Brasileira (Online) |
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Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheepMusclesRegenerationStem CellsModels, AnimalSheep.Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231Acta Cirúrgica Brasileira v.33 n.3 2018reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020180030000005info:eu-repo/semantics/openAccessBrandão,Aline Grama MoreiraConsalter,AngélicaLeite,Juliana da SilvaZamprogno,HeliaFerreira,Ana Maria Reiseng2018-04-09T00:00:00Zoai:scielo:S0102-86502018000300231Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2018-04-09T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
title |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
spellingShingle |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep Brandão,Aline Grama Moreira Muscles Regeneration Stem Cells Models, Animal Sheep. |
title_short |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
title_full |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
title_fullStr |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
title_full_unstemmed |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
title_sort |
Muscle regeneration through therapy with estromal stem cells in injury of infraespinhosus muscle of sheep |
author |
Brandão,Aline Grama Moreira |
author_facet |
Brandão,Aline Grama Moreira Consalter,Angélica Leite,Juliana da Silva Zamprogno,Helia Ferreira,Ana Maria Reis |
author_role |
author |
author2 |
Consalter,Angélica Leite,Juliana da Silva Zamprogno,Helia Ferreira,Ana Maria Reis |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Brandão,Aline Grama Moreira Consalter,Angélica Leite,Juliana da Silva Zamprogno,Helia Ferreira,Ana Maria Reis |
dc.subject.por.fl_str_mv |
Muscles Regeneration Stem Cells Models, Animal Sheep. |
topic |
Muscles Regeneration Stem Cells Models, Animal Sheep. |
description |
Abstract Purpose: To evaluate the effects of rotator cuff muscle regeneration in sheep and establish an experimental model for the use of autologous stem cells as a treatment option for tendon injuries. Methods: Infrared muscle tenotomies and Penrose drain implantation were performed on 12 shoulders of six clinically healthy adult sheep. After 60 days, the tendons were submitted to tissue repair, drainage removal, and divided into two groups according to the use of autologous stromal stem cells for treatment. Muscle regeneration was performed by biopsy on days 14 and 34 after repair. Results: The treatment group with cell therapy showed neovascularization and expressive regeneration. Complete regeneration of the muscle pattern did not occur in any sample although some muscle gain was obtained in the group 1 samples at 34 days after repair and introduction of stem cells. Fatty infiltration of these samples from group 1 at 34 days was less intense than that in samples from group 2 at 34 days after repair without the introduction of autologous precursor cells. Conclusion: The sheep proved to be a good experimental model to assist in the development of research on muscle regeneration and the autologous manipulation of stem cells as a therapeutic option. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300231 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020180030000005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.33 n.3 2018 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126444857720832 |