Does dexamethasone act in neuropeptides SP and CGRP in neurogenic inflammation of the skin? An experimental study

• Autor(a) principal: Freitas,Jose Octavio Gonçalves de
• Data de Publicação: 2015
• Outros Autores: Quieregatto,Paulo Rogério, Hochman,Bernardo, Lapin,Guilherme Abbud Franco, Mella,Stella Maria Botequio, Maximino,Jessica Ruivo, Chadi,Gerson, Ferreira,Lydia Masako
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000800523
• topic: Calcitonin Gene-Related Peptide; Substance P; Neuropeptides; Dexamethasone; Neurogenic Inflammation; Skin; Rats

PURPOSE: To investigate the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) after subcutaneous injection of dexamethasone prior to skin incision in rats.METHODS:Twenty seven Wistar-EPM-1 rats were randomly divided into three groups. The sham group (SG) of rats was injected with 0.9 % saline. The second group (Dexa) was injected with 1.0 mg/kg dexamethasone, and the third group (Dexa+) was injected with 10.0 mg/kg dexamethasone. In all groups, the three subcutaneous injections were performed 30 minutes prior to the surgical skin incision and tissue collection. SP and CGRP (15 kDa pro-CGRP and 5 kDa CGRP) were quantified by Western Blotting.RESULTS: No statistically significant differences (p>0.05) were found in pro-CGRP, CGRP and SP values in all three groups.CONCLUSION:The anti-inflammatory effect of dexamethasone did not occur when the substance P and calcitonin gene-related peptide levels were altered during the neurogenic inflammation process of skin wound healing in rats.