Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Detalhes bibliográficos
Autor(a) principal: Raymundo,Erica Calcagno
Data de Publicação: 2014
Outros Autores: Hochman,Bernardo, Nishioka,Michele Akemi, Freitas,José Octávio Gonçalves de, Maximino,Jéssica Ruivo, Chadi,Gerson, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000400224
Resumo: PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin.METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB).RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.
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spelling Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skinCarbon DioxideSubstance PCalcitonin Gene-Related PeptideNeurogenic InflammationSkinRatsPURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin.METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB).RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000400224Acta Cirúrgica Brasileira v.29 n.4 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014000400002info:eu-repo/semantics/openAccessRaymundo,Erica CalcagnoHochman,BernardoNishioka,Michele AkemiFreitas,José Octávio Gonçalves deMaximino,Jéssica RuivoChadi,GersonFerreira,Lydia Masakoeng2015-10-08T00:00:00Zoai:scielo:S0102-86502014000400224Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
title Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
spellingShingle Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
Raymundo,Erica Calcagno
Carbon Dioxide
Substance P
Calcitonin Gene-Related Peptide
Neurogenic Inflammation
Skin
Rats
title_short Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
title_full Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
title_fullStr Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
title_full_unstemmed Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
title_sort Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
author Raymundo,Erica Calcagno
author_facet Raymundo,Erica Calcagno
Hochman,Bernardo
Nishioka,Michele Akemi
Freitas,José Octávio Gonçalves de
Maximino,Jéssica Ruivo
Chadi,Gerson
Ferreira,Lydia Masako
author_role author
author2 Hochman,Bernardo
Nishioka,Michele Akemi
Freitas,José Octávio Gonçalves de
Maximino,Jéssica Ruivo
Chadi,Gerson
Ferreira,Lydia Masako
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Raymundo,Erica Calcagno
Hochman,Bernardo
Nishioka,Michele Akemi
Freitas,José Octávio Gonçalves de
Maximino,Jéssica Ruivo
Chadi,Gerson
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Carbon Dioxide
Substance P
Calcitonin Gene-Related Peptide
Neurogenic Inflammation
Skin
Rats
topic Carbon Dioxide
Substance P
Calcitonin Gene-Related Peptide
Neurogenic Inflammation
Skin
Rats
description PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin.METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB).RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000400224
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000400224
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 n.4 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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