In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000100006 |
Resumo: | Purpose: To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation. |
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Acta Cirúrgica Brasileira (Online) |
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In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblastsFibroblastsKeloidPhototherapyLasersSkinLaser Therapy, Low-LevelPurpose: To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000100006Acta Cirúrgica Brasileira v.26 n.1 2011reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502011000100006info:eu-repo/semantics/openAccessBonatti,SilvilenaHochman,BernardoTucci-Viegas,Vanina MoniqueFurtado,FabiannePinfildi,Carlos EduardoPedro,Ana CarolinaFerreira,Lydia Masakoeng2011-01-20T00:00:00Zoai:scielo:S0102-86502011000100006Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2011-01-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
title |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
spellingShingle |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts Bonatti,Silvilena Fibroblasts Keloid Phototherapy Lasers Skin Laser Therapy, Low-Level |
title_short |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
title_full |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
title_fullStr |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
title_full_unstemmed |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
title_sort |
In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts |
author |
Bonatti,Silvilena |
author_facet |
Bonatti,Silvilena Hochman,Bernardo Tucci-Viegas,Vanina Monique Furtado,Fabianne Pinfildi,Carlos Eduardo Pedro,Ana Carolina Ferreira,Lydia Masako |
author_role |
author |
author2 |
Hochman,Bernardo Tucci-Viegas,Vanina Monique Furtado,Fabianne Pinfildi,Carlos Eduardo Pedro,Ana Carolina Ferreira,Lydia Masako |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bonatti,Silvilena Hochman,Bernardo Tucci-Viegas,Vanina Monique Furtado,Fabianne Pinfildi,Carlos Eduardo Pedro,Ana Carolina Ferreira,Lydia Masako |
dc.subject.por.fl_str_mv |
Fibroblasts Keloid Phototherapy Lasers Skin Laser Therapy, Low-Level |
topic |
Fibroblasts Keloid Phototherapy Lasers Skin Laser Therapy, Low-Level |
description |
Purpose: To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000100006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502011000100006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.26 n.1 2011 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126440322629632 |