In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

Detalhes bibliográficos
Autor(a) principal: Dantas,Caroline Maria Gomes
Data de Publicação: 2010
Outros Autores: Vivan,Carolina Lapaz, Ferreira,Leila Soares, Freitas,Patricia Moreira de, Marques,Márcia Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400015
Resumo: This in vitro study aimed to analyze the effect of different parameters of phototherapy with low intensity laser on the viability of human dental pulp fibroblasts under the effect of substances released by bleaching gel. Cells were seeded into 96 wells plates (1 x 10³ cells/well) and placed in contact with culture medium conditioned by a 35 % hydrogen peroxide bleaching gel for 40 minutes, simulating the clinical condition of the in-office bleaching treatment. Cells cultured in ideal growth conditions served as positive control group (PC), and the cells grown in conditioned medium and non-irradiated served as negative control group (NC). Cells grown in conditioned medium were submitted to a single irradiation with a diode laser (40 mW, 0.04 cm²) emitting at visible red (660 nm; RL) or near infrared (780 nm; NIR) using punctual technique, in contact mode and energy densities of 4, 6 or 10 J/cm². The cell viability was analyzed through the MTT reduction assay immediately and 24 hours after the irradiation. The data was compared by ANOVA followed by the Tukey's test (p < 0.05). The cell viability increased significantly in 24 hours within each group. The PC presented cell viability significantly higher than NC in both experimental times. Only the NIR/10 J/cm² group presented cell viability similar to that of PC in 24 hours. The phototherapy with low intensity laser in defined parameters is able to compensate the cytotoxic effects of substances released by 35 % hydrogen peroxide bleaching gel.
id SBPQO-1_b5956c2cf04355e830df554e3e746a52
oai_identifier_str oai:scielo:S1806-83242010000400015
network_acronym_str SBPQO-1
network_name_str Brazilian Oral Research
repository_id_str
spelling In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gelTooth BleachingFibroblastsPhototherapyLasersThis in vitro study aimed to analyze the effect of different parameters of phototherapy with low intensity laser on the viability of human dental pulp fibroblasts under the effect of substances released by bleaching gel. Cells were seeded into 96 wells plates (1 x 10³ cells/well) and placed in contact with culture medium conditioned by a 35 % hydrogen peroxide bleaching gel for 40 minutes, simulating the clinical condition of the in-office bleaching treatment. Cells cultured in ideal growth conditions served as positive control group (PC), and the cells grown in conditioned medium and non-irradiated served as negative control group (NC). Cells grown in conditioned medium were submitted to a single irradiation with a diode laser (40 mW, 0.04 cm²) emitting at visible red (660 nm; RL) or near infrared (780 nm; NIR) using punctual technique, in contact mode and energy densities of 4, 6 or 10 J/cm². The cell viability was analyzed through the MTT reduction assay immediately and 24 hours after the irradiation. The data was compared by ANOVA followed by the Tukey's test (p < 0.05). The cell viability increased significantly in 24 hours within each group. The PC presented cell viability significantly higher than NC in both experimental times. Only the NIR/10 J/cm² group presented cell viability similar to that of PC in 24 hours. The phototherapy with low intensity laser in defined parameters is able to compensate the cytotoxic effects of substances released by 35 % hydrogen peroxide bleaching gel.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400015Brazilian Oral Research v.24 n.4 2010reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242010000400015info:eu-repo/semantics/openAccessDantas,Caroline Maria GomesVivan,Carolina LapazFerreira,Leila SoaresFreitas,Patricia Moreira deMarques,Márcia Martinseng2010-12-16T00:00:00Zoai:scielo:S1806-83242010000400015Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2010-12-16T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
title In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
spellingShingle In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
Dantas,Caroline Maria Gomes
Tooth Bleaching
Fibroblasts
Phototherapy
Lasers
title_short In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
title_full In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
title_fullStr In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
title_full_unstemmed In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
title_sort In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel
author Dantas,Caroline Maria Gomes
author_facet Dantas,Caroline Maria Gomes
Vivan,Carolina Lapaz
Ferreira,Leila Soares
Freitas,Patricia Moreira de
Marques,Márcia Martins
author_role author
author2 Vivan,Carolina Lapaz
Ferreira,Leila Soares
Freitas,Patricia Moreira de
Marques,Márcia Martins
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dantas,Caroline Maria Gomes
Vivan,Carolina Lapaz
Ferreira,Leila Soares
Freitas,Patricia Moreira de
Marques,Márcia Martins
dc.subject.por.fl_str_mv Tooth Bleaching
Fibroblasts
Phototherapy
Lasers
topic Tooth Bleaching
Fibroblasts
Phototherapy
Lasers
description This in vitro study aimed to analyze the effect of different parameters of phototherapy with low intensity laser on the viability of human dental pulp fibroblasts under the effect of substances released by bleaching gel. Cells were seeded into 96 wells plates (1 x 10³ cells/well) and placed in contact with culture medium conditioned by a 35 % hydrogen peroxide bleaching gel for 40 minutes, simulating the clinical condition of the in-office bleaching treatment. Cells cultured in ideal growth conditions served as positive control group (PC), and the cells grown in conditioned medium and non-irradiated served as negative control group (NC). Cells grown in conditioned medium were submitted to a single irradiation with a diode laser (40 mW, 0.04 cm²) emitting at visible red (660 nm; RL) or near infrared (780 nm; NIR) using punctual technique, in contact mode and energy densities of 4, 6 or 10 J/cm². The cell viability was analyzed through the MTT reduction assay immediately and 24 hours after the irradiation. The data was compared by ANOVA followed by the Tukey's test (p < 0.05). The cell viability increased significantly in 24 hours within each group. The PC presented cell viability significantly higher than NC in both experimental times. Only the NIR/10 J/cm² group presented cell viability similar to that of PC in 24 hours. The phototherapy with low intensity laser in defined parameters is able to compensate the cytotoxic effects of substances released by 35 % hydrogen peroxide bleaching gel.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242010000400015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.24 n.4 2010
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
_version_ 1750318322104139776