Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung

Detalhes bibliográficos
Autor(a) principal: Quemelo,Paulo Roberto Veiga
Data de Publicação: 2010
Outros Autores: Peres,Luiz Cesar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400005
Resumo: PURPOSE: To investigate the proliferation and neuronal death in brain tissue heterotopia in the lung in an experimental model during both fetal and neonatal periods. METHODS: Twenty four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation (PBI) were collected on the 18th gestational day (group E18) and six other on the 8th postnatal day (group P8). Immunohistochemical staining for PCNA and Bcl2 were used to assess proliferation and cell death. RESULTS: PCNA Labelling Index (LI) in heterotopic brain tissue was greater in fetal than postnatal period (E18 &gt; P8) (p<0.05) and the immunostaining with Bcl2 antibody did not show difference. CONCLUSION: Cell proliferation is maintained in brain tissue heterotopia, although apoptosis is also observed.
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spelling Proliferation and cell death in an experimental model of brain tissue heterotopia in the lungCell ProliferationCell DeathMicePURPOSE: To investigate the proliferation and neuronal death in brain tissue heterotopia in the lung in an experimental model during both fetal and neonatal periods. METHODS: Twenty four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation (PBI) were collected on the 18th gestational day (group E18) and six other on the 8th postnatal day (group P8). Immunohistochemical staining for PCNA and Bcl2 were used to assess proliferation and cell death. RESULTS: PCNA Labelling Index (LI) in heterotopic brain tissue was greater in fetal than postnatal period (E18 &gt; P8) (p<0.05) and the immunostaining with Bcl2 antibody did not show difference. CONCLUSION: Cell proliferation is maintained in brain tissue heterotopia, although apoptosis is also observed.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400005Acta Cirúrgica Brasileira v.25 n.4 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000400005info:eu-repo/semantics/openAccessQuemelo,Paulo Roberto VeigaPeres,Luiz Cesareng2010-07-26T00:00:00Zoai:scielo:S0102-86502010000400005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-07-26T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
title Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
spellingShingle Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
Quemelo,Paulo Roberto Veiga
Cell Proliferation
Cell Death
Mice
title_short Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
title_full Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
title_fullStr Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
title_full_unstemmed Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
title_sort Proliferation and cell death in an experimental model of brain tissue heterotopia in the lung
author Quemelo,Paulo Roberto Veiga
author_facet Quemelo,Paulo Roberto Veiga
Peres,Luiz Cesar
author_role author
author2 Peres,Luiz Cesar
author2_role author
dc.contributor.author.fl_str_mv Quemelo,Paulo Roberto Veiga
Peres,Luiz Cesar
dc.subject.por.fl_str_mv Cell Proliferation
Cell Death
Mice
topic Cell Proliferation
Cell Death
Mice
description PURPOSE: To investigate the proliferation and neuronal death in brain tissue heterotopia in the lung in an experimental model during both fetal and neonatal periods. METHODS: Twenty four pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation (PBI) were collected on the 18th gestational day (group E18) and six other on the 8th postnatal day (group P8). Immunohistochemical staining for PCNA and Bcl2 were used to assess proliferation and cell death. RESULTS: PCNA Labelling Index (LI) in heterotopic brain tissue was greater in fetal than postnatal period (E18 &gt; P8) (p<0.05) and the immunostaining with Bcl2 antibody did not show difference. CONCLUSION: Cell proliferation is maintained in brain tissue heterotopia, although apoptosis is also observed.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502010000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.25 n.4 2010
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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