Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202 |
Resumo: | ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane. |
id |
SBDPC-1_945345ffe1817100f109178ddd897eca |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502021001000202 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbitsOrthopedicsInfrared RaysLow-Level Light TherapyRabbitsABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202Acta Cirúrgica Brasileira v.36 n.10 2021reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/acb361003info:eu-repo/semantics/openAccessCoris,Jeniffer Gabriela FigueroaRahal,Sheila CaneveseFonseca-Alves,Carlos EduardoMamprim,Maria JaquelineInamassu,Letícia RochaSampaio,Alexandre Botelho de AbreuKano,Washington TakashiMesquita,Luciane dos ReisSilva Junior,José Ivaldo de Siqueiraeng2021-11-19T00:00:00Zoai:scielo:S0102-86502021001000202Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2021-11-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
title |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
spellingShingle |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits Coris,Jeniffer Gabriela Figueroa Orthopedics Infrared Rays Low-Level Light Therapy Rabbits |
title_short |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
title_full |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
title_fullStr |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
title_full_unstemmed |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
title_sort |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
author |
Coris,Jeniffer Gabriela Figueroa |
author_facet |
Coris,Jeniffer Gabriela Figueroa Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira |
author_role |
author |
author2 |
Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Coris,Jeniffer Gabriela Figueroa Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira |
dc.subject.por.fl_str_mv |
Orthopedics Infrared Rays Low-Level Light Therapy Rabbits |
topic |
Orthopedics Infrared Rays Low-Level Light Therapy Rabbits |
description |
ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/acb361003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.36 n.10 2021 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126446308950016 |