Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/ACB361003 http://hdl.handle.net/11449/229952 |
Resumo: | Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusion: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane. |
id |
UNSP_3af578498300355f4f2040c7e0ba0e69 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229952 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbitsInfrared raysLow-level light therapyOrthopedicsRabbitsPurpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusion: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Animal Biotechnology-Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP)Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP)Animal Biotechnology-Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP)Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP)CAPES: 001FAPESP: 2019/01750-0Universidade Estadual Paulista (UNESP)Coris, Jeniffer Gabriela Figueroa [UNESP]Rahal, Sheila Canevese [UNESP]Fonseca-Alves, Carlos Eduardo [UNESP]Mamprim, Maria Jaqueline [UNESP]Inamassu, Letícia Rocha [UNESP]de Abreu Sampaio, Alexandre Botelho [UNESP]Kano, Washington Takashi [UNESP]Mesquita, Luciane Dos Reis [UNESP]Junior, José Ivaldo de Siqueira Silva [UNESP]2022-04-29T08:36:47Z2022-04-29T08:36:47Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/ACB361003Acta Cirurgica Brasileira, v. 36, n. 10, 2021.1678-26740102-8650http://hdl.handle.net/11449/22995210.1590/ACB3610032-s2.0-85119937278Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccess2024-09-09T14:06:04Zoai:repositorio.unesp.br:11449/229952Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
title |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
spellingShingle |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits Coris, Jeniffer Gabriela Figueroa [UNESP] Infrared rays Low-level light therapy Orthopedics Rabbits |
title_short |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
title_full |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
title_fullStr |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
title_full_unstemmed |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
title_sort |
Effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
author |
Coris, Jeniffer Gabriela Figueroa [UNESP] |
author_facet |
Coris, Jeniffer Gabriela Figueroa [UNESP] Rahal, Sheila Canevese [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Mamprim, Maria Jaqueline [UNESP] Inamassu, Letícia Rocha [UNESP] de Abreu Sampaio, Alexandre Botelho [UNESP] Kano, Washington Takashi [UNESP] Mesquita, Luciane Dos Reis [UNESP] Junior, José Ivaldo de Siqueira Silva [UNESP] |
author_role |
author |
author2 |
Rahal, Sheila Canevese [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Mamprim, Maria Jaqueline [UNESP] Inamassu, Letícia Rocha [UNESP] de Abreu Sampaio, Alexandre Botelho [UNESP] Kano, Washington Takashi [UNESP] Mesquita, Luciane Dos Reis [UNESP] Junior, José Ivaldo de Siqueira Silva [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Coris, Jeniffer Gabriela Figueroa [UNESP] Rahal, Sheila Canevese [UNESP] Fonseca-Alves, Carlos Eduardo [UNESP] Mamprim, Maria Jaqueline [UNESP] Inamassu, Letícia Rocha [UNESP] de Abreu Sampaio, Alexandre Botelho [UNESP] Kano, Washington Takashi [UNESP] Mesquita, Luciane Dos Reis [UNESP] Junior, José Ivaldo de Siqueira Silva [UNESP] |
dc.subject.por.fl_str_mv |
Infrared rays Low-level light therapy Orthopedics Rabbits |
topic |
Infrared rays Low-level light therapy Orthopedics Rabbits |
description |
Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusion: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:36:47Z 2022-04-29T08:36:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/ACB361003 Acta Cirurgica Brasileira, v. 36, n. 10, 2021. 1678-2674 0102-8650 http://hdl.handle.net/11449/229952 10.1590/ACB361003 2-s2.0-85119937278 |
url |
http://dx.doi.org/10.1590/ACB361003 http://hdl.handle.net/11449/229952 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 36, n. 10, 2021. 1678-2674 0102-8650 10.1590/ACB361003 2-s2.0-85119937278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021345878081536 |