Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008 |
Resumo: | PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury. |
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Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in ratsAcetylcysteineLungShock, HemorrhagicCell DeathRatsPURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008Acta Cirúrgica Brasileira v.27 n.8 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000800008info:eu-repo/semantics/openAccessSaad,Paulo FernandesSaad,Karen RuggeriOliveira Filho,Luiz Dantas deFerreira,Sueli GomesKoike,Marcia KiyomiMontero,Edna Frasson de Souzaeng2012-07-25T00:00:00Zoai:scielo:S0102-86502012000800008Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-07-25T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
title |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
spellingShingle |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats Saad,Paulo Fernandes Acetylcysteine Lung Shock, Hemorrhagic Cell Death Rats |
title_short |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
title_full |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
title_fullStr |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
title_full_unstemmed |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
title_sort |
Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats |
author |
Saad,Paulo Fernandes |
author_facet |
Saad,Paulo Fernandes Saad,Karen Ruggeri Oliveira Filho,Luiz Dantas de Ferreira,Sueli Gomes Koike,Marcia Kiyomi Montero,Edna Frasson de Souza |
author_role |
author |
author2 |
Saad,Karen Ruggeri Oliveira Filho,Luiz Dantas de Ferreira,Sueli Gomes Koike,Marcia Kiyomi Montero,Edna Frasson de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Saad,Paulo Fernandes Saad,Karen Ruggeri Oliveira Filho,Luiz Dantas de Ferreira,Sueli Gomes Koike,Marcia Kiyomi Montero,Edna Frasson de Souza |
dc.subject.por.fl_str_mv |
Acetylcysteine Lung Shock, Hemorrhagic Cell Death Rats |
topic |
Acetylcysteine Lung Shock, Hemorrhagic Cell Death Rats |
description |
PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012000800008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.8 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441299902464 |