Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

Detalhes bibliográficos
Autor(a) principal: Saad,Paulo Fernandes
Data de Publicação: 2012
Outros Autores: Saad,Karen Ruggeri, Oliveira Filho,Luiz Dantas de, Ferreira,Sueli Gomes, Koike,Marcia Kiyomi, Montero,Edna Frasson de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008
Resumo: PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.
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spelling Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in ratsAcetylcysteineLungShock, HemorrhagicCell DeathRatsPURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008Acta Cirúrgica Brasileira v.27 n.8 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000800008info:eu-repo/semantics/openAccessSaad,Paulo FernandesSaad,Karen RuggeriOliveira Filho,Luiz Dantas deFerreira,Sueli GomesKoike,Marcia KiyomiMontero,Edna Frasson de Souzaeng2012-07-25T00:00:00Zoai:scielo:S0102-86502012000800008Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-07-25T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
title Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
spellingShingle Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
Saad,Paulo Fernandes
Acetylcysteine
Lung
Shock, Hemorrhagic
Cell Death
Rats
title_short Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
title_full Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
title_fullStr Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
title_full_unstemmed Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
title_sort Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats
author Saad,Paulo Fernandes
author_facet Saad,Paulo Fernandes
Saad,Karen Ruggeri
Oliveira Filho,Luiz Dantas de
Ferreira,Sueli Gomes
Koike,Marcia Kiyomi
Montero,Edna Frasson de Souza
author_role author
author2 Saad,Karen Ruggeri
Oliveira Filho,Luiz Dantas de
Ferreira,Sueli Gomes
Koike,Marcia Kiyomi
Montero,Edna Frasson de Souza
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Saad,Paulo Fernandes
Saad,Karen Ruggeri
Oliveira Filho,Luiz Dantas de
Ferreira,Sueli Gomes
Koike,Marcia Kiyomi
Montero,Edna Frasson de Souza
dc.subject.por.fl_str_mv Acetylcysteine
Lung
Shock, Hemorrhagic
Cell Death
Rats
topic Acetylcysteine
Lung
Shock, Hemorrhagic
Cell Death
Rats
description PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000800008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502012000800008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.27 n.8 2012
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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