Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

• Autor(a) principal: Saad, Paulo Fernandes [UNIFESP]
• Data de Publicação: 2012
• Outros Autores: Saad, Karen Ruggeri [UNIFESP], Oliveira Filho, Luiz Dantas de, Ferreira, Sueli Gomes, Koike, Marcia Kiyomi [UNIFESP], Montero, Edna Frasson de Souza [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502012000800008; http://repositorio.unifesp.br/handle/11600/41982
• topic: Acetylcysteine; Lung; Shock, Hemorrhagic; Cell Death; Rats

PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS).METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3.RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group.CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.