Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205 |
Resumo: | Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture. |
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Acta Cirúrgica Brasileira (Online) |
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Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hoursCytokinesHypothermiaInflammationLiverRats Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205Acta Cirúrgica Brasileira v.35 n.2 2020reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020200020000005info:eu-repo/semantics/openAccessGrezzana Filho,Tomaz de Jesus MariaLongo,LarisseSantos,Jorge Luiz dosGabiatti,GemersonBoffil,CarlosSantos,Emanuel Burck dosCerski,Carlos Thadeu SchmidtChedid,Marcio FernandesCorso,Carlos Otavioeng2020-06-05T00:00:00Zoai:scielo:S0102-86502020000200205Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2020-06-05T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
title |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
spellingShingle |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours Grezzana Filho,Tomaz de Jesus Maria Cytokines Hypothermia Inflammation Liver Rats |
title_short |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
title_full |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
title_fullStr |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
title_full_unstemmed |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
title_sort |
Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours |
author |
Grezzana Filho,Tomaz de Jesus Maria |
author_facet |
Grezzana Filho,Tomaz de Jesus Maria Longo,Larisse Santos,Jorge Luiz dos Gabiatti,Gemerson Boffil,Carlos Santos,Emanuel Burck dos Cerski,Carlos Thadeu Schmidt Chedid,Marcio Fernandes Corso,Carlos Otavio |
author_role |
author |
author2 |
Longo,Larisse Santos,Jorge Luiz dos Gabiatti,Gemerson Boffil,Carlos Santos,Emanuel Burck dos Cerski,Carlos Thadeu Schmidt Chedid,Marcio Fernandes Corso,Carlos Otavio |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Grezzana Filho,Tomaz de Jesus Maria Longo,Larisse Santos,Jorge Luiz dos Gabiatti,Gemerson Boffil,Carlos Santos,Emanuel Burck dos Cerski,Carlos Thadeu Schmidt Chedid,Marcio Fernandes Corso,Carlos Otavio |
dc.subject.por.fl_str_mv |
Cytokines Hypothermia Inflammation Liver Rats |
topic |
Cytokines Hypothermia Inflammation Liver Rats |
description |
Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020200020000005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.35 n.2 2020 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126445755301888 |