Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours

Detalhes bibliográficos
Autor(a) principal: Grezzana Filho,Tomaz de Jesus Maria
Data de Publicação: 2020
Outros Autores: Longo,Larisse, Santos,Jorge Luiz dos, Gabiatti,Gemerson, Boffil,Carlos, Santos,Emanuel Burck dos, Cerski,Carlos Thadeu Schmidt, Chedid,Marcio Fernandes, Corso,Carlos Otavio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205
Resumo: Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.
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spelling Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hoursCytokinesHypothermiaInflammationLiverRats Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205Acta Cirúrgica Brasileira v.35 n.2 2020reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020200020000005info:eu-repo/semantics/openAccessGrezzana Filho,Tomaz de Jesus MariaLongo,LarisseSantos,Jorge Luiz dosGabiatti,GemersonBoffil,CarlosSantos,Emanuel Burck dosCerski,Carlos Thadeu SchmidtChedid,Marcio FernandesCorso,Carlos Otavioeng2020-06-05T00:00:00Zoai:scielo:S0102-86502020000200205Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2020-06-05T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
title Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
spellingShingle Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
Grezzana Filho,Tomaz de Jesus Maria
Cytokines
Hypothermia
Inflammation
Liver
Rats
title_short Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
title_full Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
title_fullStr Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
title_full_unstemmed Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
title_sort Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours
author Grezzana Filho,Tomaz de Jesus Maria
author_facet Grezzana Filho,Tomaz de Jesus Maria
Longo,Larisse
Santos,Jorge Luiz dos
Gabiatti,Gemerson
Boffil,Carlos
Santos,Emanuel Burck dos
Cerski,Carlos Thadeu Schmidt
Chedid,Marcio Fernandes
Corso,Carlos Otavio
author_role author
author2 Longo,Larisse
Santos,Jorge Luiz dos
Gabiatti,Gemerson
Boffil,Carlos
Santos,Emanuel Burck dos
Cerski,Carlos Thadeu Schmidt
Chedid,Marcio Fernandes
Corso,Carlos Otavio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Grezzana Filho,Tomaz de Jesus Maria
Longo,Larisse
Santos,Jorge Luiz dos
Gabiatti,Gemerson
Boffil,Carlos
Santos,Emanuel Burck dos
Cerski,Carlos Thadeu Schmidt
Chedid,Marcio Fernandes
Corso,Carlos Otavio
dc.subject.por.fl_str_mv Cytokines
Hypothermia
Inflammation
Liver
Rats
topic Cytokines
Hypothermia
Inflammation
Liver
Rats
description Purpose To investigate the effects of induction of selective liver hypothermia in a rodent model. Methods Seven male Wistar rats were subjected to 90 minutes of partial 70% liver ischemia and topic liver 26°C hypothermia (H group). Other seven male Wistar rats were subjected to 90 minutes of partial 70% normothermic liver ischemia (N group). Five additional rats underwent a midline incision and section of liver ligaments under normothermic conditions and without any liver ischemia (sham group). All animals were sacrificed 24-h after reperfusion, and livers were sampled for analyses. Pathology sections were scored for sinusoidal congestion, ballooning, hepatocelllular necrosis and the presence of neutrophilic infiltrates. Results At the end of the experiment, liver tissue expressions of TNF-ɑ, IL-1β, iNOS and TNF-ɑ/IL-10 ratio were significantly reduced in the H group compared to N group, whereas IL-10 and eNOS were significantly increased in H group. Histopathological injury scores revealed a significant decrease in ischemia/reperfusion (I/R) injuries in H group. Conclusion Selective liver hypothermia prevented I/R injury by inhibiting the release of inflammatory cytokines, preserves microcirculation, prevents hepatocellular necrosis and leukocyte infiltration, allowing maintenance of the liver architecture.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000200205
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020200020000005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.35 n.2 2020
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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