Photodynamic inactivation of in vitro bacterial cultures from pressure ulcers

• Autor(a) principal: Carvalho,Paulo de Tarso Camillo de
• Data de Publicação: 2006
• Outros Autores: Marques,Ana Paula da Costa, Reis,Felipe Abdalla dos, Belchior,Ana Carulina Guimarães, Silva,Iandara Schettert, Habitante,Carlos Alexandre, Sussai,Daniela Aparecida
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006001000008
• topic: Photosensitizing Agents; Anti-Bacterial Agents; Skin Ulcers

PURPOSE: To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers. METHODS: Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blood agar for the growth of Gram-positive bacteria, and MacConkey agar for Gram-negative bacteria, and incubated for 48 hours. The results obtained from counting the colony-forming units were correlated and subjected to statistical analysis, adopting the significance level of p > or = 0.05. RESULTS: From analysis of variance (ANOVA), the result for the general mean was p = 0.0215. Using the t test with post-hoc test, the result for TBO vs. Control was p = 0.0186, and for TBO + Laser vs. Control it was p = 0.0039. CONCLUSION: There was a significant reduction in colony-forming units when the cultures were subjected to photodynamic therapy.