Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/978-3-030-70601-2_173 http://hdl.handle.net/11449/239953 |
Resumo: | The colonisation of the pilosebaceous follicle by Propionibacterium acnes (P. acnes) is known as one of the main factors driving acne by taking part in the inflammatory response of the skin. Antimicrobial Photodynamic Therapy (aPDT) has important applicability in skin diseases, however its use with hypericin (Hypericum perforatum) photosensitizer for inhibit of P. acnes have not yet been clarified. The aim of this study was to evaluate in vitro the effects of aPDT using hypericin photosensitizer associated with red low-level laser therapy on P. acnes biofilms. The biofilms were placed in 96-well microplates by using standard suspensions (2 × 107 CFU/mL) and grown in BHI broth for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min (C); laser groups received irradiation of energies of 3 J (L3J); hypericin group at concentrations of 15 μg/ml for 3 min (H15%); aPDT group received 15 μg/ml concentration of hypericin associated with laser energy of 3 J (H15%L3J). After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms in H15% and H15%L3J. In addition, this reduction was higher in H15%L3J. This study showed that hypericin and aPDT using hypericin photosensitizer showed effective antimicrobial action for inhibiting P. acnes biofilms and may be promising resources in the clinical treatment of acne vulgaris. |
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Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum)Acne VulgarisAntimicrobial photodynamic therapyHypericinPhotochemotherapyPhotosensitizing agentsPropionibacteriumThe colonisation of the pilosebaceous follicle by Propionibacterium acnes (P. acnes) is known as one of the main factors driving acne by taking part in the inflammatory response of the skin. Antimicrobial Photodynamic Therapy (aPDT) has important applicability in skin diseases, however its use with hypericin (Hypericum perforatum) photosensitizer for inhibit of P. acnes have not yet been clarified. The aim of this study was to evaluate in vitro the effects of aPDT using hypericin photosensitizer associated with red low-level laser therapy on P. acnes biofilms. The biofilms were placed in 96-well microplates by using standard suspensions (2 × 107 CFU/mL) and grown in BHI broth for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min (C); laser groups received irradiation of energies of 3 J (L3J); hypericin group at concentrations of 15 μg/ml for 3 min (H15%); aPDT group received 15 μg/ml concentration of hypericin associated with laser energy of 3 J (H15%L3J). After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms in H15% and H15%L3J. In addition, this reduction was higher in H15%L3J. This study showed that hypericin and aPDT using hypericin photosensitizer showed effective antimicrobial action for inhibiting P. acnes biofilms and may be promising resources in the clinical treatment of acne vulgaris.Scientific and Technological Institute of Brasil Univesity Brasil University, Carolina Fonseca, 235, ItaqueraInstitute of Science and Technology Department of Biosciences and Oral Diagnosis Universidade Estadual Paulista Júlio de Mesquita FilhoDentistry Graduate Program Universidade Cruzeiro do SulInstitute of Science and Technology Department of Biosciences and Oral Diagnosis Universidade Estadual Paulista Júlio de Mesquita FilhoBrasil UniversityUniversidade Estadual Paulista (UNESP)Universidade Cruzeiro do SulBarroso, R. A.Navarro, R.Tim, C. R.Ramos, L. P. [UNESP]Oliveira, L. D. de [UNESP]Araki, A. T.Macedo, D. B.Fernandes, K. G. CamaraAssis, L.2023-03-01T19:54:57Z2023-03-01T19:54:57Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1153-1156http://dx.doi.org/10.1007/978-3-030-70601-2_173IFMBE Proceedings, v. 83, p. 1153-1156.1433-92771680-0737http://hdl.handle.net/11449/23995310.1007/978-3-030-70601-2_1732-s2.0-85128982683Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIFMBE Proceedingsinfo:eu-repo/semantics/openAccess2023-03-01T19:54:58Zoai:repositorio.unesp.br:11449/239953Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T19:54:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
title |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
spellingShingle |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) Barroso, R. A. Acne Vulgaris Antimicrobial photodynamic therapy Hypericin Photochemotherapy Photosensitizing agents Propionibacterium |
title_short |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
title_full |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
title_fullStr |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
title_full_unstemmed |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
title_sort |
Effect of Photodynamic Inactivation of Propionibacterium Acnes Biofilms by Hypericin (Hypericum perforatum) |
author |
Barroso, R. A. |
author_facet |
Barroso, R. A. Navarro, R. Tim, C. R. Ramos, L. P. [UNESP] Oliveira, L. D. de [UNESP] Araki, A. T. Macedo, D. B. Fernandes, K. G. Camara Assis, L. |
author_role |
author |
author2 |
Navarro, R. Tim, C. R. Ramos, L. P. [UNESP] Oliveira, L. D. de [UNESP] Araki, A. T. Macedo, D. B. Fernandes, K. G. Camara Assis, L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Brasil University Universidade Estadual Paulista (UNESP) Universidade Cruzeiro do Sul |
dc.contributor.author.fl_str_mv |
Barroso, R. A. Navarro, R. Tim, C. R. Ramos, L. P. [UNESP] Oliveira, L. D. de [UNESP] Araki, A. T. Macedo, D. B. Fernandes, K. G. Camara Assis, L. |
dc.subject.por.fl_str_mv |
Acne Vulgaris Antimicrobial photodynamic therapy Hypericin Photochemotherapy Photosensitizing agents Propionibacterium |
topic |
Acne Vulgaris Antimicrobial photodynamic therapy Hypericin Photochemotherapy Photosensitizing agents Propionibacterium |
description |
The colonisation of the pilosebaceous follicle by Propionibacterium acnes (P. acnes) is known as one of the main factors driving acne by taking part in the inflammatory response of the skin. Antimicrobial Photodynamic Therapy (aPDT) has important applicability in skin diseases, however its use with hypericin (Hypericum perforatum) photosensitizer for inhibit of P. acnes have not yet been clarified. The aim of this study was to evaluate in vitro the effects of aPDT using hypericin photosensitizer associated with red low-level laser therapy on P. acnes biofilms. The biofilms were placed in 96-well microplates by using standard suspensions (2 × 107 CFU/mL) and grown in BHI broth for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min (C); laser groups received irradiation of energies of 3 J (L3J); hypericin group at concentrations of 15 μg/ml for 3 min (H15%); aPDT group received 15 μg/ml concentration of hypericin associated with laser energy of 3 J (H15%L3J). After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms in H15% and H15%L3J. In addition, this reduction was higher in H15%L3J. This study showed that hypericin and aPDT using hypericin photosensitizer showed effective antimicrobial action for inhibiting P. acnes biofilms and may be promising resources in the clinical treatment of acne vulgaris. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T19:54:57Z 2023-03-01T19:54:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/978-3-030-70601-2_173 IFMBE Proceedings, v. 83, p. 1153-1156. 1433-9277 1680-0737 http://hdl.handle.net/11449/239953 10.1007/978-3-030-70601-2_173 2-s2.0-85128982683 |
url |
http://dx.doi.org/10.1007/978-3-030-70601-2_173 http://hdl.handle.net/11449/239953 |
identifier_str_mv |
IFMBE Proceedings, v. 83, p. 1153-1156. 1433-9277 1680-0737 10.1007/978-3-030-70601-2_173 2-s2.0-85128982683 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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IFMBE Proceedings |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1153-1156 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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