Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001300005 |
Resumo: | PURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac®, PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p<0.05. RESULTS: The established protocol with less mortality and increased number of NEC was with Esbilac® at a concentration of 0.19 g/ml of diet instilled volume of 0.1 ml, every 3 hours, for 72 hours. All infants fed with artificial milk lost weight. In the degree score of intestinal injury, the ESB, IH and NEC groups were considered positive for NEC with greater histological injury in the latter. CONCLUSION: The described NEC protocol in rats allowed a greater survival of puppies with a greater number of animals affected by the disease. |
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Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedureEnterocolitis, NecrotizingIschemiaIntestine, Small/injuriesMethodologyRatsPURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac®, PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p<0.05. RESULTS: The established protocol with less mortality and increased number of NEC was with Esbilac® at a concentration of 0.19 g/ml of diet instilled volume of 0.1 ml, every 3 hours, for 72 hours. All infants fed with artificial milk lost weight. In the degree score of intestinal injury, the ESB, IH and NEC groups were considered positive for NEC with greater histological injury in the latter. CONCLUSION: The described NEC protocol in rats allowed a greater survival of puppies with a greater number of animals affected by the disease.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001300005Acta Cirúrgica Brasileira v.28 suppl.1 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013001300005info:eu-repo/semantics/openAccessGonçalves,Frances Lilian LanhellasGallindo,Rodrigo MeloSoares,Lucas Manoel MangueiraFigueira,Rebeca LopesVolpe,Fábio Antônio PerecimPereira-da-Silva,Maurício AndréSbragia,Lourençoeng2013-02-01T00:00:00Zoai:scielo:S0102-86502013001300005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-02-01T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
title |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
spellingShingle |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure Gonçalves,Frances Lilian Lanhellas Enterocolitis, Necrotizing Ischemia Intestine, Small/injuries Methodology Rats |
title_short |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
title_full |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
title_fullStr |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
title_full_unstemmed |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
title_sort |
Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure |
author |
Gonçalves,Frances Lilian Lanhellas |
author_facet |
Gonçalves,Frances Lilian Lanhellas Gallindo,Rodrigo Melo Soares,Lucas Manoel Mangueira Figueira,Rebeca Lopes Volpe,Fábio Antônio Perecim Pereira-da-Silva,Maurício André Sbragia,Lourenço |
author_role |
author |
author2 |
Gallindo,Rodrigo Melo Soares,Lucas Manoel Mangueira Figueira,Rebeca Lopes Volpe,Fábio Antônio Perecim Pereira-da-Silva,Maurício André Sbragia,Lourenço |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves,Frances Lilian Lanhellas Gallindo,Rodrigo Melo Soares,Lucas Manoel Mangueira Figueira,Rebeca Lopes Volpe,Fábio Antônio Perecim Pereira-da-Silva,Maurício André Sbragia,Lourenço |
dc.subject.por.fl_str_mv |
Enterocolitis, Necrotizing Ischemia Intestine, Small/injuries Methodology Rats |
topic |
Enterocolitis, Necrotizing Ischemia Intestine, Small/injuries Methodology Rats |
description |
PURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac®, PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p<0.05. RESULTS: The established protocol with less mortality and increased number of NEC was with Esbilac® at a concentration of 0.19 g/ml of diet instilled volume of 0.1 ml, every 3 hours, for 72 hours. All infants fed with artificial milk lost weight. In the degree score of intestinal injury, the ESB, IH and NEC groups were considered positive for NEC with greater histological injury in the latter. CONCLUSION: The described NEC protocol in rats allowed a greater survival of puppies with a greater number of animals affected by the disease. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001300005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001300005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502013001300005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.28 suppl.1 2013 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
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1752126442175463424 |