Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study

Detalhes bibliográficos
Autor(a) principal: Silva,Silvana Marques e
Data de Publicação: 2015
Outros Autores: Jerônimo,Márcio Sousa, Silva-Pereira,Ildinete da, Tavares,Aldo Henrique, Bocca,Anamélia Lorenzetti, Sousa,João Batista de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015001100762
Resumo: PURPOSE : To evaluate the effects of metoclopramide on metalloproteinases (MMP) and interleukins (IL) gene expression in colonic anastomoses in rats. METHODS : Eighty rats were divided into two groups for euthanasia on the 3rd or 7th postoperative day (POD), then into two subgroups for sepsis induction or not, and then into subgroups to receive either metoclopramide or saline solution. Left colonic anastomosis were performed and then analyzed. RESULTS : On the 3rd POD, metoclopramide was associated with increased expression of MMP-1a, MMP-13, and TNF-α. On the 7th POD, the transcripts of all MMPs, TNF-α, IL-1β, IFN-γ, and IL-10 of the treated animals became negatively modulated. In the presence of sepsis, metoclopramide did not change MMPs and decreased IL-6, IL-1β, IFN-γ and IL-10 gene expression on the 3rd POD. On the 7th POD, increased expression of all MMPs, IFN-γ and IL-10 and negative modulated TNF-α and IL-6 gene expression. CONCLUSION : Administration of metoclopramide increased metalloproteinases and interleukins gene expression on the 3rd postoperative day and negatively modulated them on the 7th POD. In the presence of abdominal sepsis, metoclopramide did not change MMPs and decreased ILs gene expression on the 3rd POD. On the 7th POD, the drug increased expression of all MMPs.
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spelling Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental studyInterleukinsMatrix MetalloproteinasesMetoclopramideAnastomosis, SurgicalRats PURPOSE : To evaluate the effects of metoclopramide on metalloproteinases (MMP) and interleukins (IL) gene expression in colonic anastomoses in rats. METHODS : Eighty rats were divided into two groups for euthanasia on the 3rd or 7th postoperative day (POD), then into two subgroups for sepsis induction or not, and then into subgroups to receive either metoclopramide or saline solution. Left colonic anastomosis were performed and then analyzed. RESULTS : On the 3rd POD, metoclopramide was associated with increased expression of MMP-1a, MMP-13, and TNF-α. On the 7th POD, the transcripts of all MMPs, TNF-α, IL-1β, IFN-γ, and IL-10 of the treated animals became negatively modulated. In the presence of sepsis, metoclopramide did not change MMPs and decreased IL-6, IL-1β, IFN-γ and IL-10 gene expression on the 3rd POD. On the 7th POD, increased expression of all MMPs, IFN-γ and IL-10 and negative modulated TNF-α and IL-6 gene expression. CONCLUSION : Administration of metoclopramide increased metalloproteinases and interleukins gene expression on the 3rd postoperative day and negatively modulated them on the 7th POD. In the presence of abdominal sepsis, metoclopramide did not change MMPs and decreased ILs gene expression on the 3rd POD. On the 7th POD, the drug increased expression of all MMPs.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2015-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015001100762Acta Cirúrgica Brasileira v.30 n.11 2015reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-865020150110000007info:eu-repo/semantics/openAccessSilva,Silvana Marques eJerônimo,Márcio SousaSilva-Pereira,Ildinete daTavares,Aldo HenriqueBocca,Anamélia LorenzettiSousa,João Batista deeng2015-12-02T00:00:00Zoai:scielo:S0102-86502015001100762Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-12-02T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
title Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
spellingShingle Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
Silva,Silvana Marques e
Interleukins
Matrix Metalloproteinases
Metoclopramide
Anastomosis, Surgical
Rats
title_short Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
title_full Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
title_fullStr Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
title_full_unstemmed Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
title_sort Effects of metoclopramide on the expression of metalloproteinases and interleukins in left colonic anastomoses. An experimental study
author Silva,Silvana Marques e
author_facet Silva,Silvana Marques e
Jerônimo,Márcio Sousa
Silva-Pereira,Ildinete da
Tavares,Aldo Henrique
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
author_role author
author2 Jerônimo,Márcio Sousa
Silva-Pereira,Ildinete da
Tavares,Aldo Henrique
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Silvana Marques e
Jerônimo,Márcio Sousa
Silva-Pereira,Ildinete da
Tavares,Aldo Henrique
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
dc.subject.por.fl_str_mv Interleukins
Matrix Metalloproteinases
Metoclopramide
Anastomosis, Surgical
Rats
topic Interleukins
Matrix Metalloproteinases
Metoclopramide
Anastomosis, Surgical
Rats
description PURPOSE : To evaluate the effects of metoclopramide on metalloproteinases (MMP) and interleukins (IL) gene expression in colonic anastomoses in rats. METHODS : Eighty rats were divided into two groups for euthanasia on the 3rd or 7th postoperative day (POD), then into two subgroups for sepsis induction or not, and then into subgroups to receive either metoclopramide or saline solution. Left colonic anastomosis were performed and then analyzed. RESULTS : On the 3rd POD, metoclopramide was associated with increased expression of MMP-1a, MMP-13, and TNF-α. On the 7th POD, the transcripts of all MMPs, TNF-α, IL-1β, IFN-γ, and IL-10 of the treated animals became negatively modulated. In the presence of sepsis, metoclopramide did not change MMPs and decreased IL-6, IL-1β, IFN-γ and IL-10 gene expression on the 3rd POD. On the 7th POD, increased expression of all MMPs, IFN-γ and IL-10 and negative modulated TNF-α and IL-6 gene expression. CONCLUSION : Administration of metoclopramide increased metalloproteinases and interleukins gene expression on the 3rd postoperative day and negatively modulated them on the 7th POD. In the presence of abdominal sepsis, metoclopramide did not change MMPs and decreased ILs gene expression on the 3rd POD. On the 7th POD, the drug increased expression of all MMPs.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015001100762
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015001100762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-865020150110000007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.30 n.11 2015
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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