Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

Detalhes bibliográficos
Autor(a) principal: Gouveia,Eliana Maria Ferreira
Data de Publicação: 2011
Outros Autores: Silva,Iandara Schettert, Nakazato,Gerson, Araujo,Flábio Ribeiro de, Chang,Marilene Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000200013
Resumo: PURPOSE: To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. METHODS: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). RESULTS: All infected animals presented diarrhea and had the gene eae amplified by PCR. CONCLUSION: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria.
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spelling Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pupsEnteropathogenic Escherichia coliVirulence FactorsPolymerase Chain ReactionDogsPURPOSE: To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. METHODS: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). RESULTS: All infected animals presented diarrhea and had the gene eae amplified by PCR. CONCLUSION: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000200013Acta Cirúrgica Brasileira v.26 n.2 2011reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502011000200013info:eu-repo/semantics/openAccessGouveia,Eliana Maria FerreiraSilva,Iandara SchettertNakazato,GersonAraujo,Flábio Ribeiro deChang,Marilene Rodrigueseng2011-04-01T00:00:00Zoai:scielo:S0102-86502011000200013Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2011-04-01T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
title Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
spellingShingle Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
Gouveia,Eliana Maria Ferreira
Enteropathogenic Escherichia coli
Virulence Factors
Polymerase Chain Reaction
Dogs
title_short Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
title_full Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
title_fullStr Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
title_full_unstemmed Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
title_sort Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups
author Gouveia,Eliana Maria Ferreira
author_facet Gouveia,Eliana Maria Ferreira
Silva,Iandara Schettert
Nakazato,Gerson
Araujo,Flábio Ribeiro de
Chang,Marilene Rodrigues
author_role author
author2 Silva,Iandara Schettert
Nakazato,Gerson
Araujo,Flábio Ribeiro de
Chang,Marilene Rodrigues
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gouveia,Eliana Maria Ferreira
Silva,Iandara Schettert
Nakazato,Gerson
Araujo,Flábio Ribeiro de
Chang,Marilene Rodrigues
dc.subject.por.fl_str_mv Enteropathogenic Escherichia coli
Virulence Factors
Polymerase Chain Reaction
Dogs
topic Enteropathogenic Escherichia coli
Virulence Factors
Polymerase Chain Reaction
Dogs
description PURPOSE: To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. METHODS: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). RESULTS: All infected animals presented diarrhea and had the gene eae amplified by PCR. CONCLUSION: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000200013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000200013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502011000200013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.26 n.2 2011
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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