Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research on Biomedical Engineering (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402018000300254 |
Resumo: | Abstract Introduction The use of the same imaging and quantification techniques in small animals and clinical studies presents the opportunity for direct translational research in drug discovery and development, in neuropharmacological basis of neurological and psychiatric diseases, and in optimization of drug therapy. Thus, positron emission tomography (PET) studies in rodents can bridge the gap between pre-clinical and clinical research. The aim should be to find a method with capability to measure, without compromising accuracy, glucose distribution in the structures of the brain, which can also be used in pathological situations and with applicability for other substances than glucose analogue. Methods This is a systematic review of several assessment techniques available, including visual and quantitative methods that enable the investigation of the transport mechanisms and enzymes involved in glucose metabolism in the brain. In addition to the ex vivo methods, PET with glucose analogues allows in vivo analyses using qualitative, semiquantitative and quantitative methods. Results These techniques provide different results, and the applicability of a specific method is related to the purpose of the study and the multiple factors that may interfere in the process. Conclusion This review provides a solid background of tools and quantification methods for medical physicists and other professionals interested in cerebral glycolytic metabolism quantification in experimental animals. It also addresses the main factors related to animals, equipment and techniques that are used, as well as how these factors should be understood to better interpret the results obtained from experiments. |
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Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animalsNuclear medicineQuantificationPositron emission tomographyMetabolismFDGBrainAbstract Introduction The use of the same imaging and quantification techniques in small animals and clinical studies presents the opportunity for direct translational research in drug discovery and development, in neuropharmacological basis of neurological and psychiatric diseases, and in optimization of drug therapy. Thus, positron emission tomography (PET) studies in rodents can bridge the gap between pre-clinical and clinical research. The aim should be to find a method with capability to measure, without compromising accuracy, glucose distribution in the structures of the brain, which can also be used in pathological situations and with applicability for other substances than glucose analogue. Methods This is a systematic review of several assessment techniques available, including visual and quantitative methods that enable the investigation of the transport mechanisms and enzymes involved in glucose metabolism in the brain. In addition to the ex vivo methods, PET with glucose analogues allows in vivo analyses using qualitative, semiquantitative and quantitative methods. Results These techniques provide different results, and the applicability of a specific method is related to the purpose of the study and the multiple factors that may interfere in the process. Conclusion This review provides a solid background of tools and quantification methods for medical physicists and other professionals interested in cerebral glycolytic metabolism quantification in experimental animals. It also addresses the main factors related to animals, equipment and techniques that are used, as well as how these factors should be understood to better interpret the results obtained from experiments.Sociedade Brasileira de Engenharia Biomédica2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402018000300254Research on Biomedical Engineering v.34 n.3 2018reponame:Research on Biomedical Engineering (Online)instname:Sociedade Brasileira de Engenharia Biomédica (SBEB)instacron:SBEB10.1590/2446-4740.04517info:eu-repo/semantics/openAccessPrando,SilvanaOno,Carla RachelRobilotta,Cecil ChowSapienza,Marcelo Tatiteng2018-10-30T00:00:00Zoai:scielo:S2446-47402018000300254Revistahttp://www.rbejournal.org/https://old.scielo.br/oai/scielo-oai.php||rbe@rbejournal.org2446-47402446-4732opendoar:2018-10-30T00:00Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB)false |
dc.title.none.fl_str_mv |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
title |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
spellingShingle |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals Prando,Silvana Nuclear medicine Quantification Positron emission tomography Metabolism FDG Brain |
title_short |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
title_full |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
title_fullStr |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
title_full_unstemmed |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
title_sort |
Methods for quantification of cerebral glycolytic metabolism using 2-deoxy-2-[18 F]fluoroglucose in small animals |
author |
Prando,Silvana |
author_facet |
Prando,Silvana Ono,Carla Rachel Robilotta,Cecil Chow Sapienza,Marcelo Tatit |
author_role |
author |
author2 |
Ono,Carla Rachel Robilotta,Cecil Chow Sapienza,Marcelo Tatit |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Prando,Silvana Ono,Carla Rachel Robilotta,Cecil Chow Sapienza,Marcelo Tatit |
dc.subject.por.fl_str_mv |
Nuclear medicine Quantification Positron emission tomography Metabolism FDG Brain |
topic |
Nuclear medicine Quantification Positron emission tomography Metabolism FDG Brain |
description |
Abstract Introduction The use of the same imaging and quantification techniques in small animals and clinical studies presents the opportunity for direct translational research in drug discovery and development, in neuropharmacological basis of neurological and psychiatric diseases, and in optimization of drug therapy. Thus, positron emission tomography (PET) studies in rodents can bridge the gap between pre-clinical and clinical research. The aim should be to find a method with capability to measure, without compromising accuracy, glucose distribution in the structures of the brain, which can also be used in pathological situations and with applicability for other substances than glucose analogue. Methods This is a systematic review of several assessment techniques available, including visual and quantitative methods that enable the investigation of the transport mechanisms and enzymes involved in glucose metabolism in the brain. In addition to the ex vivo methods, PET with glucose analogues allows in vivo analyses using qualitative, semiquantitative and quantitative methods. Results These techniques provide different results, and the applicability of a specific method is related to the purpose of the study and the multiple factors that may interfere in the process. Conclusion This review provides a solid background of tools and quantification methods for medical physicists and other professionals interested in cerebral glycolytic metabolism quantification in experimental animals. It also addresses the main factors related to animals, equipment and techniques that are used, as well as how these factors should be understood to better interpret the results obtained from experiments. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402018000300254 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2446-47402018000300254 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2446-4740.04517 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Engenharia Biomédica |
dc.source.none.fl_str_mv |
Research on Biomedical Engineering v.34 n.3 2018 reponame:Research on Biomedical Engineering (Online) instname:Sociedade Brasileira de Engenharia Biomédica (SBEB) instacron:SBEB |
instname_str |
Sociedade Brasileira de Engenharia Biomédica (SBEB) |
instacron_str |
SBEB |
institution |
SBEB |
reponame_str |
Research on Biomedical Engineering (Online) |
collection |
Research on Biomedical Engineering (Online) |
repository.name.fl_str_mv |
Research on Biomedical Engineering (Online) - Sociedade Brasileira de Engenharia Biomédica (SBEB) |
repository.mail.fl_str_mv |
||rbe@rbejournal.org |
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1752126288991092736 |