Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients

Detalhes bibliográficos
Autor(a) principal: Lobo Júnior,João Paulo
Data de Publicação: 2017
Outros Autores: Brescansin,Catiane Pompilio, Santos-Weiss,Izabella C. R., Welter,Marciane, Souza,Emanuel Maltempi de, Rego,Fabiane Gomes de Moraes, Picheth,Geraldo, Alberton,Dayane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos de Endocrinologia e Metabolismo (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233
Resumo: ABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.
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spelling Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patientsDiabetes screeninggestational diabetesfluorescent advanced glycation end productsABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.Sociedade Brasileira de Endocrinologia e Metabologia2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233Archives of Endocrinology and Metabolism v.61 n.3 2017reponame:Arquivos de Endocrinologia e Metabolismo (Online)instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)instacron:SBEM10.1590/2359-3997000000238info:eu-repo/semantics/openAccessLobo Júnior,João PauloBrescansin,Catiane PompilioSantos-Weiss,Izabella C. R.Welter,MarcianeSouza,Emanuel Maltempi deRego,Fabiane Gomes de MoraesPicheth,GeraldoAlberton,Dayaneeng2017-07-05T00:00:00Zoai:scielo:S2359-39972017000300233Revistahttps://www.aem-sbem.com/https://old.scielo.br/oai/scielo-oai.php||aem.editorial.office@endocrino.org.br2359-42922359-3997opendoar:2017-07-05T00:00Arquivos de Endocrinologia e Metabolismo (Online) - Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)false
dc.title.none.fl_str_mv Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
title Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
spellingShingle Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
Lobo Júnior,João Paulo
Diabetes screening
gestational diabetes
fluorescent advanced glycation end products
title_short Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
title_full Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
title_fullStr Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
title_full_unstemmed Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
title_sort Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
author Lobo Júnior,João Paulo
author_facet Lobo Júnior,João Paulo
Brescansin,Catiane Pompilio
Santos-Weiss,Izabella C. R.
Welter,Marciane
Souza,Emanuel Maltempi de
Rego,Fabiane Gomes de Moraes
Picheth,Geraldo
Alberton,Dayane
author_role author
author2 Brescansin,Catiane Pompilio
Santos-Weiss,Izabella C. R.
Welter,Marciane
Souza,Emanuel Maltempi de
Rego,Fabiane Gomes de Moraes
Picheth,Geraldo
Alberton,Dayane
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lobo Júnior,João Paulo
Brescansin,Catiane Pompilio
Santos-Weiss,Izabella C. R.
Welter,Marciane
Souza,Emanuel Maltempi de
Rego,Fabiane Gomes de Moraes
Picheth,Geraldo
Alberton,Dayane
dc.subject.por.fl_str_mv Diabetes screening
gestational diabetes
fluorescent advanced glycation end products
topic Diabetes screening
gestational diabetes
fluorescent advanced glycation end products
description ABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2359-3997000000238
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
dc.source.none.fl_str_mv Archives of Endocrinology and Metabolism v.61 n.3 2017
reponame:Arquivos de Endocrinologia e Metabolismo (Online)
instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)
instacron:SBEM
instname_str Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)
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institution SBEM
reponame_str Arquivos de Endocrinologia e Metabolismo (Online)
collection Arquivos de Endocrinologia e Metabolismo (Online)
repository.name.fl_str_mv Arquivos de Endocrinologia e Metabolismo (Online) - Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)
repository.mail.fl_str_mv ||aem.editorial.office@endocrino.org.br
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