Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos de Endocrinologia e Metabolismo (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233 |
Resumo: | ABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations. |
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Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patientsDiabetes screeninggestational diabetesfluorescent advanced glycation end productsABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.Sociedade Brasileira de Endocrinologia e Metabologia2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233Archives of Endocrinology and Metabolism v.61 n.3 2017reponame:Arquivos de Endocrinologia e Metabolismo (Online)instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)instacron:SBEM10.1590/2359-3997000000238info:eu-repo/semantics/openAccessLobo Júnior,João PauloBrescansin,Catiane PompilioSantos-Weiss,Izabella C. R.Welter,MarcianeSouza,Emanuel Maltempi deRego,Fabiane Gomes de MoraesPicheth,GeraldoAlberton,Dayaneeng2017-07-05T00:00:00Zoai:scielo:S2359-39972017000300233Revistahttps://www.aem-sbem.com/https://old.scielo.br/oai/scielo-oai.php||aem.editorial.office@endocrino.org.br2359-42922359-3997opendoar:2017-07-05T00:00Arquivos de Endocrinologia e Metabolismo (Online) - Sociedade Brasileira de Endocrinologia e Metabologia (SBEM)false |
dc.title.none.fl_str_mv |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
title |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
spellingShingle |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients Lobo Júnior,João Paulo Diabetes screening gestational diabetes fluorescent advanced glycation end products |
title_short |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
title_full |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
title_fullStr |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
title_full_unstemmed |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
title_sort |
Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients |
author |
Lobo Júnior,João Paulo |
author_facet |
Lobo Júnior,João Paulo Brescansin,Catiane Pompilio Santos-Weiss,Izabella C. R. Welter,Marciane Souza,Emanuel Maltempi de Rego,Fabiane Gomes de Moraes Picheth,Geraldo Alberton,Dayane |
author_role |
author |
author2 |
Brescansin,Catiane Pompilio Santos-Weiss,Izabella C. R. Welter,Marciane Souza,Emanuel Maltempi de Rego,Fabiane Gomes de Moraes Picheth,Geraldo Alberton,Dayane |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lobo Júnior,João Paulo Brescansin,Catiane Pompilio Santos-Weiss,Izabella C. R. Welter,Marciane Souza,Emanuel Maltempi de Rego,Fabiane Gomes de Moraes Picheth,Geraldo Alberton,Dayane |
dc.subject.por.fl_str_mv |
Diabetes screening gestational diabetes fluorescent advanced glycation end products |
topic |
Diabetes screening gestational diabetes fluorescent advanced glycation end products |
description |
ABSTRACT Objectives Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Subjects and methods Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). Results No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. Conclusion It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972017000300233 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2359-3997000000238 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Endocrinologia e Metabologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Endocrinologia e Metabologia |
dc.source.none.fl_str_mv |
Archives of Endocrinology and Metabolism v.61 n.3 2017 reponame:Arquivos de Endocrinologia e Metabolismo (Online) instname:Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) instacron:SBEM |
instname_str |
Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) |
instacron_str |
SBEM |
institution |
SBEM |
reponame_str |
Arquivos de Endocrinologia e Metabolismo (Online) |
collection |
Arquivos de Endocrinologia e Metabolismo (Online) |
repository.name.fl_str_mv |
Arquivos de Endocrinologia e Metabolismo (Online) - Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) |
repository.mail.fl_str_mv |
||aem.editorial.office@endocrino.org.br |
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1752122514964742144 |