Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fitopatologia Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008 |
Resumo: | Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection. |
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Fitopatologia Brasileira |
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Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in BrazilClosteroviridaeAmpelovirusGLRaV-3Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.Sociedade Brasileira de Fitopatologia2007-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008Fitopatologia Brasileira v.32 n.4 2007reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582007000400008info:eu-repo/semantics/openAccessFajardo,Thor V.M.Dianese,Érico C.Eiras,MarceloCerqueira,Daniela M.Lopes,Daniela B.Ferreira,Marisa A.S.V.Martins,Cláudia R.F.eng2007-10-19T00:00:00Zoai:scielo:S0100-41582007000400008Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2007-10-19T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false |
dc.title.none.fl_str_mv |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
spellingShingle |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil Fajardo,Thor V.M. Closteroviridae Ampelovirus GLRaV-3 |
title_short |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_full |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_fullStr |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_full_unstemmed |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_sort |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
author |
Fajardo,Thor V.M. |
author_facet |
Fajardo,Thor V.M. Dianese,Érico C. Eiras,Marcelo Cerqueira,Daniela M. Lopes,Daniela B. Ferreira,Marisa A.S.V. Martins,Cláudia R.F. |
author_role |
author |
author2 |
Dianese,Érico C. Eiras,Marcelo Cerqueira,Daniela M. Lopes,Daniela B. Ferreira,Marisa A.S.V. Martins,Cláudia R.F. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fajardo,Thor V.M. Dianese,Érico C. Eiras,Marcelo Cerqueira,Daniela M. Lopes,Daniela B. Ferreira,Marisa A.S.V. Martins,Cláudia R.F. |
dc.subject.por.fl_str_mv |
Closteroviridae Ampelovirus GLRaV-3 |
topic |
Closteroviridae Ampelovirus GLRaV-3 |
description |
Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-41582007000400008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Fitopatologia Brasileira v.32 n.4 2007 reponame:Fitopatologia Brasileira instname:Sociedade Brasileira de Fitopatologia (SBF) instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia (SBF) |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Fitopatologia Brasileira |
collection |
Fitopatologia Brasileira |
repository.name.fl_str_mv |
Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF) |
repository.mail.fl_str_mv |
||sbf-revista@ufla.br |
_version_ |
1754734651007565824 |