Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil

Detalhes bibliográficos
Autor(a) principal: Fajardo,Thor V.M.
Data de Publicação: 2007
Outros Autores: Dianese,Érico C., Eiras,Marcelo, Cerqueira,Daniela M., Lopes,Daniela B., Ferreira,Marisa A.S.V., Martins,Cláudia R.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Fitopatologia Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008
Resumo: Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.
id SBF-3_4f4c0b1a126ed2d53ffaff3f2ebe08b6
oai_identifier_str oai:scielo:S0100-41582007000400008
network_acronym_str SBF-3
network_name_str Fitopatologia Brasileira
repository_id_str
spelling Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in BrazilClosteroviridaeAmpelovirusGLRaV-3Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.Sociedade Brasileira de Fitopatologia2007-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008Fitopatologia Brasileira v.32 n.4 2007reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582007000400008info:eu-repo/semantics/openAccessFajardo,Thor V.M.Dianese,Érico C.Eiras,MarceloCerqueira,Daniela M.Lopes,Daniela B.Ferreira,Marisa A.S.V.Martins,Cláudia R.F.eng2007-10-19T00:00:00Zoai:scielo:S0100-41582007000400008Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2007-10-19T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false
dc.title.none.fl_str_mv Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
spellingShingle Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
Fajardo,Thor V.M.
Closteroviridae
Ampelovirus
GLRaV-3
title_short Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_fullStr Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full_unstemmed Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_sort Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
author Fajardo,Thor V.M.
author_facet Fajardo,Thor V.M.
Dianese,Érico C.
Eiras,Marcelo
Cerqueira,Daniela M.
Lopes,Daniela B.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
author_role author
author2 Dianese,Érico C.
Eiras,Marcelo
Cerqueira,Daniela M.
Lopes,Daniela B.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fajardo,Thor V.M.
Dianese,Érico C.
Eiras,Marcelo
Cerqueira,Daniela M.
Lopes,Daniela B.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
dc.subject.por.fl_str_mv Closteroviridae
Ampelovirus
GLRaV-3
topic Closteroviridae
Ampelovirus
GLRaV-3
description Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.
publishDate 2007
dc.date.none.fl_str_mv 2007-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-41582007000400008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Fitopatologia Brasileira v.32 n.4 2007
reponame:Fitopatologia Brasileira
instname:Sociedade Brasileira de Fitopatologia (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia (SBF)
instacron_str SBF
institution SBF
reponame_str Fitopatologia Brasileira
collection Fitopatologia Brasileira
repository.name.fl_str_mv Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)
repository.mail.fl_str_mv ||sbf-revista@ufla.br
_version_ 1754734651007565824