Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/26887 https://dx.doi.org/10.1590/S0100-41582007000400008 |
Resumo: | Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection. |
id |
UNB_0030e89b7075dbfb207aceb3722e7d16 |
---|---|
oai_identifier_str |
oai:repositorio.unb.br:10482/26887 |
network_acronym_str |
UNB |
network_name_str |
Repositório Institucional da UnB |
repository_id_str |
|
spelling |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in BrazilVariabilidade do gene da proteína capsidial do Grapevine leafroll-associated virus 3 no BrasilClosteroviridaeAmpelovírusGLRaV-3Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.O enrolamento da folha é uma doença economicamente importante que afeta videiras (Vitis spp.). Nove vírus sorologicamente distintos, Grapevine leafroll-associated virus-1 a -9, estão associados à doença. Este estudo descreve a seqüência do gene da proteína capsidial de quatro isolados do GLRaV-3 encontrados no Vale do Rio São Francisco, Nordeste do Brasil. O RNA viral foi extraído de plantas positivas em ELISA para o GLRaV-3 e o gene da proteína capsidial completo foi amplificado por RT-PCR. As seqüências foram geradas automaticamente e comparadas a seqüências completas do gene da proteína capsidial de isolados Norte-Americano (NY1) e Chineses (Dawanhong Nº;2 e SL10) de GLRaV-3. Os quatro isolados estudados, denominados Pet-1 a 4, exibiram identidades de aminoácidos deduzidos de 98-100% (Pet-1 a 3) e 95% (Pet-4) com os isolados Norte-Americano e Chineses. Um total de dezessete substituições de aminoácidos foi detectado entre os quatro isolados caracterizados em comparação com as seqüências do NY1, Dawanhong No.2 e SL10. Os resultados indicaram a existência de variação natural entre os isolados de GLRaV-3 de videiras, demonstrando também a falta de correlação entre dados de sequência e origem geográfica. Esta variabilidade deve ser considerada quando se selecionam regiões do genoma viral para uma detecção molecular confiável e consistente.Em processamentoSociedade Brasileira de Fitopatologia2017-12-07T04:47:27Z2017-12-07T04:47:27Z2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFitopatol. bras.,v.32,n.4,p.335-340,2007http://repositorio.unb.br/handle/10482/26887https://dx.doi.org/10.1590/S0100-41582007000400008Fajardo, Thor V.M.Dianese, Érico C.Eiras, MarceloCerqueira, Daniela M.Lopes, Daniela B.Ferreira, Marisa A.S.V.Martins, Cláudia R.F.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:11Zoai:repositorio.unb.br:10482/26887Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:11Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil Variabilidade do gene da proteína capsidial do Grapevine leafroll-associated virus 3 no Brasil |
title |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
spellingShingle |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil Fajardo, Thor V.M. Closteroviridae Ampelovírus GLRaV-3 |
title_short |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_full |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_fullStr |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_full_unstemmed |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
title_sort |
Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil |
author |
Fajardo, Thor V.M. |
author_facet |
Fajardo, Thor V.M. Dianese, Érico C. Eiras, Marcelo Cerqueira, Daniela M. Lopes, Daniela B. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. |
author_role |
author |
author2 |
Dianese, Érico C. Eiras, Marcelo Cerqueira, Daniela M. Lopes, Daniela B. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fajardo, Thor V.M. Dianese, Érico C. Eiras, Marcelo Cerqueira, Daniela M. Lopes, Daniela B. Ferreira, Marisa A.S.V. Martins, Cláudia R.F. |
dc.subject.por.fl_str_mv |
Closteroviridae Ampelovírus GLRaV-3 |
topic |
Closteroviridae Ampelovírus GLRaV-3 |
description |
Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2017-12-07T04:47:27Z 2017-12-07T04:47:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Fitopatol. bras.,v.32,n.4,p.335-340,2007 http://repositorio.unb.br/handle/10482/26887 https://dx.doi.org/10.1590/S0100-41582007000400008 |
identifier_str_mv |
Fitopatol. bras.,v.32,n.4,p.335-340,2007 |
url |
http://repositorio.unb.br/handle/10482/26887 https://dx.doi.org/10.1590/S0100-41582007000400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
_version_ |
1814508205258047488 |