Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil

Detalhes bibliográficos
Autor(a) principal: Fajardo, Thor V.M.
Data de Publicação: 2007
Outros Autores: Dianese, Érico C., Eiras, Marcelo, Cerqueira, Daniela M., Lopes, Daniela B., Ferreira, Marisa A.S.V., Martins, Cláudia R.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/26887
https://dx.doi.org/10.1590/S0100-41582007000400008
Resumo: Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.
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spelling Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in BrazilVariabilidade do gene da proteína capsidial do Grapevine leafroll-associated virus 3 no BrasilClosteroviridaeAmpelovírusGLRaV-3Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.O enrolamento da folha é uma doença economicamente importante que afeta videiras (Vitis spp.). Nove vírus sorologicamente distintos, Grapevine leafroll-associated virus-1 a -9, estão associados à doença. Este estudo descreve a seqüência do gene da proteína capsidial de quatro isolados do GLRaV-3 encontrados no Vale do Rio São Francisco, Nordeste do Brasil. O RNA viral foi extraído de plantas positivas em ELISA para o GLRaV-3 e o gene da proteína capsidial completo foi amplificado por RT-PCR. As seqüências foram geradas automaticamente e comparadas a seqüências completas do gene da proteína capsidial de isolados Norte-Americano (NY1) e Chineses (Dawanhong Nº;2 e SL10) de GLRaV-3. Os quatro isolados estudados, denominados Pet-1 a 4, exibiram identidades de aminoácidos deduzidos de 98-100% (Pet-1 a 3) e 95% (Pet-4) com os isolados Norte-Americano e Chineses. Um total de dezessete substituições de aminoácidos foi detectado entre os quatro isolados caracterizados em comparação com as seqüências do NY1, Dawanhong No.2 e SL10. Os resultados indicaram a existência de variação natural entre os isolados de GLRaV-3 de videiras, demonstrando também a falta de correlação entre dados de sequência e origem geográfica. Esta variabilidade deve ser considerada quando se selecionam regiões do genoma viral para uma detecção molecular confiável e consistente.Em processamentoSociedade Brasileira de Fitopatologia2017-12-07T04:47:27Z2017-12-07T04:47:27Z2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFitopatol. bras.,v.32,n.4,p.335-340,2007http://repositorio.unb.br/handle/10482/26887https://dx.doi.org/10.1590/S0100-41582007000400008Fajardo, Thor V.M.Dianese, Érico C.Eiras, MarceloCerqueira, Daniela M.Lopes, Daniela B.Ferreira, Marisa A.S.V.Martins, Cláudia R.F.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:11Zoai:repositorio.unb.br:10482/26887Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:11Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
Variabilidade do gene da proteína capsidial do Grapevine leafroll-associated virus 3 no Brasil
title Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
spellingShingle Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
Fajardo, Thor V.M.
Closteroviridae
Ampelovírus
GLRaV-3
title_short Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_fullStr Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_full_unstemmed Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
title_sort Variability of the coat protein gene of Grapevine leafroll-associated virus 3 in Brazil
author Fajardo, Thor V.M.
author_facet Fajardo, Thor V.M.
Dianese, Érico C.
Eiras, Marcelo
Cerqueira, Daniela M.
Lopes, Daniela B.
Ferreira, Marisa A.S.V.
Martins, Cláudia R.F.
author_role author
author2 Dianese, Érico C.
Eiras, Marcelo
Cerqueira, Daniela M.
Lopes, Daniela B.
Ferreira, Marisa A.S.V.
Martins, Cláudia R.F.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fajardo, Thor V.M.
Dianese, Érico C.
Eiras, Marcelo
Cerqueira, Daniela M.
Lopes, Daniela B.
Ferreira, Marisa A.S.V.
Martins, Cláudia R.F.
dc.subject.por.fl_str_mv Closteroviridae
Ampelovírus
GLRaV-3
topic Closteroviridae
Ampelovírus
GLRaV-3
description Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and 95% (Pet-4) with North American and Chinese isolates. A total of seventeen amino acid substitutions was detected among the four characterized isolates in comparison to the NY1, Dawanhong No.2 and SL10 sequences. The results indicated the existence of natural variation among GLRaV-3 isolates from grapevines, also demonstrating a lack of correlation between sequence data and geographic origin. This variability should be considered when selecting regions of the viral genome targeted for reliable and consistent virus molecular detection.
publishDate 2007
dc.date.none.fl_str_mv 2007
2017-12-07T04:47:27Z
2017-12-07T04:47:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Fitopatol. bras.,v.32,n.4,p.335-340,2007
http://repositorio.unb.br/handle/10482/26887
https://dx.doi.org/10.1590/S0100-41582007000400008
identifier_str_mv Fitopatol. bras.,v.32,n.4,p.335-340,2007
url http://repositorio.unb.br/handle/10482/26887
https://dx.doi.org/10.1590/S0100-41582007000400008
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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