Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000200007 |
Resumo: | Fungi are disease-causing agents in plants and affect crops of economic importance. One control method is to induce resistance in the host by using biological control with hypovirulent phytopathogenic fungi. Here, we report the detection of a mycovirus in a strain of Colletotrichum gloeosporioides causing anthracnose of cashew tree. The strain C. gloeosporioides URM 4903 was isolated from a cashew tree (Anacardium occidentale) in Igarassu, PE, Brazil. After nucleic acid extraction and electrophoresis, the band corresponding to a possible double-stranded RNA (dsRNA) was purified by cellulose column chromatography. Nine extrachromosomal bands were obtained. Enzymatic digestion with DNAse I and Nuclease S1 had no effect on these bands, indicating their dsRNA nature. Transmission electron microscopic examination of extracts from this strain showed the presence of isometric particles (30-35 nm in diameter). These data strongly suggest the infection of this C. gloeosporioides strain by a dsRNA mycovirus. Once the hypovirulence of this strain is confirmed, the strain may be used for the biological control of cashew anthracnose. |
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Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew treeAnacardium occidentaleanthracnosebiological controlhypovirulencemycovirusphytopathogenic fungiFungi are disease-causing agents in plants and affect crops of economic importance. One control method is to induce resistance in the host by using biological control with hypovirulent phytopathogenic fungi. Here, we report the detection of a mycovirus in a strain of Colletotrichum gloeosporioides causing anthracnose of cashew tree. The strain C. gloeosporioides URM 4903 was isolated from a cashew tree (Anacardium occidentale) in Igarassu, PE, Brazil. After nucleic acid extraction and electrophoresis, the band corresponding to a possible double-stranded RNA (dsRNA) was purified by cellulose column chromatography. Nine extrachromosomal bands were obtained. Enzymatic digestion with DNAse I and Nuclease S1 had no effect on these bands, indicating their dsRNA nature. Transmission electron microscopic examination of extracts from this strain showed the presence of isometric particles (30-35 nm in diameter). These data strongly suggest the infection of this C. gloeosporioides strain by a dsRNA mycovirus. Once the hypovirulence of this strain is confirmed, the strain may be used for the biological control of cashew anthracnose.Sociedade Brasileira de Fitopatologia2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000200007Tropical Plant Pathology v.37 n.2 2012reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762012000200007info:eu-repo/semantics/openAccessFigueirêdo,Lívio C. deFigueirêdo,Girlene S. deGiancoli,Ágata C.H.Tanaka,Francisco A.O.Silva,Leonor A.O. daKitajima,Elliot W.A. Filho,SpartacoAzevedo,João Lúcioeng2012-05-18T00:00:00Zoai:scielo:S1982-56762012000200007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2012-05-18T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
title |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
spellingShingle |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree Figueirêdo,Lívio C. de Anacardium occidentale anthracnose biological control hypovirulence mycovirus phytopathogenic fungi |
title_short |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
title_full |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
title_fullStr |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
title_full_unstemmed |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
title_sort |
Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree |
author |
Figueirêdo,Lívio C. de |
author_facet |
Figueirêdo,Lívio C. de Figueirêdo,Girlene S. de Giancoli,Ágata C.H. Tanaka,Francisco A.O. Silva,Leonor A.O. da Kitajima,Elliot W. A. Filho,Spartaco Azevedo,João Lúcio |
author_role |
author |
author2 |
Figueirêdo,Girlene S. de Giancoli,Ágata C.H. Tanaka,Francisco A.O. Silva,Leonor A.O. da Kitajima,Elliot W. A. Filho,Spartaco Azevedo,João Lúcio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Figueirêdo,Lívio C. de Figueirêdo,Girlene S. de Giancoli,Ágata C.H. Tanaka,Francisco A.O. Silva,Leonor A.O. da Kitajima,Elliot W. A. Filho,Spartaco Azevedo,João Lúcio |
dc.subject.por.fl_str_mv |
Anacardium occidentale anthracnose biological control hypovirulence mycovirus phytopathogenic fungi |
topic |
Anacardium occidentale anthracnose biological control hypovirulence mycovirus phytopathogenic fungi |
description |
Fungi are disease-causing agents in plants and affect crops of economic importance. One control method is to induce resistance in the host by using biological control with hypovirulent phytopathogenic fungi. Here, we report the detection of a mycovirus in a strain of Colletotrichum gloeosporioides causing anthracnose of cashew tree. The strain C. gloeosporioides URM 4903 was isolated from a cashew tree (Anacardium occidentale) in Igarassu, PE, Brazil. After nucleic acid extraction and electrophoresis, the band corresponding to a possible double-stranded RNA (dsRNA) was purified by cellulose column chromatography. Nine extrachromosomal bands were obtained. Enzymatic digestion with DNAse I and Nuclease S1 had no effect on these bands, indicating their dsRNA nature. Transmission electron microscopic examination of extracts from this strain showed the presence of isometric particles (30-35 nm in diameter). These data strongly suggest the infection of this C. gloeosporioides strain by a dsRNA mycovirus. Once the hypovirulence of this strain is confirmed, the strain may be used for the biological control of cashew anthracnose. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762012000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762012000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.37 n.2 2012 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585834921984 |