Genetic variability of Bipolaris sorokiniana isolates using URP-PCR

Detalhes bibliográficos
Autor(a) principal: Mann,Michele B.
Data de Publicação: 2014
Outros Autores: Spadari,Cristina C., Feltrin,Thaisa, Frazzon,Ana Paula G., Germani,José Carlos, Sand,Sueli T. Van Der
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007
Resumo: Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana.
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spelling Genetic variability of Bipolaris sorokiniana isolates using URP-PCRTriticum aestivunspot blotch diseasewheatSpot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana.Sociedade Brasileira de Fitopatologia2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007Tropical Plant Pathology v.39 n.2 2014reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762014000200007info:eu-repo/semantics/openAccessMann,Michele B.Spadari,Cristina C.Feltrin,ThaisaFrazzon,Ana Paula G.Germani,José CarlosSand,Sueli T. Van Dereng2014-04-16T00:00:00Zoai:scielo:S1982-56762014000200007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2014-04-16T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
title Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
spellingShingle Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
Mann,Michele B.
Triticum aestivun
spot blotch disease
wheat
title_short Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
title_full Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
title_fullStr Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
title_full_unstemmed Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
title_sort Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
author Mann,Michele B.
author_facet Mann,Michele B.
Spadari,Cristina C.
Feltrin,Thaisa
Frazzon,Ana Paula G.
Germani,José Carlos
Sand,Sueli T. Van Der
author_role author
author2 Spadari,Cristina C.
Feltrin,Thaisa
Frazzon,Ana Paula G.
Germani,José Carlos
Sand,Sueli T. Van Der
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mann,Michele B.
Spadari,Cristina C.
Feltrin,Thaisa
Frazzon,Ana Paula G.
Germani,José Carlos
Sand,Sueli T. Van Der
dc.subject.por.fl_str_mv Triticum aestivun
spot blotch disease
wheat
topic Triticum aestivun
spot blotch disease
wheat
description Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762014000200007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.39 n.2 2014
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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