Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007 |
Resumo: | Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana. |
id |
SBF-6_55b035eb44aaf41dafd7f08109ea6185 |
---|---|
oai_identifier_str |
oai:scielo:S1982-56762014000200007 |
network_acronym_str |
SBF-6 |
network_name_str |
Tropical plant pathology (Online) |
repository_id_str |
|
spelling |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCRTriticum aestivunspot blotch diseasewheatSpot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana.Sociedade Brasileira de Fitopatologia2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007Tropical Plant Pathology v.39 n.2 2014reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762014000200007info:eu-repo/semantics/openAccessMann,Michele B.Spadari,Cristina C.Feltrin,ThaisaFrazzon,Ana Paula G.Germani,José CarlosSand,Sueli T. Van Dereng2014-04-16T00:00:00Zoai:scielo:S1982-56762014000200007Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2014-04-16T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
spellingShingle |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR Mann,Michele B. Triticum aestivun spot blotch disease wheat |
title_short |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_full |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_fullStr |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_full_unstemmed |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_sort |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
author |
Mann,Michele B. |
author_facet |
Mann,Michele B. Spadari,Cristina C. Feltrin,Thaisa Frazzon,Ana Paula G. Germani,José Carlos Sand,Sueli T. Van Der |
author_role |
author |
author2 |
Spadari,Cristina C. Feltrin,Thaisa Frazzon,Ana Paula G. Germani,José Carlos Sand,Sueli T. Van Der |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mann,Michele B. Spadari,Cristina C. Feltrin,Thaisa Frazzon,Ana Paula G. Germani,José Carlos Sand,Sueli T. Van Der |
dc.subject.por.fl_str_mv |
Triticum aestivun spot blotch disease wheat |
topic |
Triticum aestivun spot blotch disease wheat |
description |
Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP's are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762014000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.39 n.2 2014 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824586367598592 |