Genetic variability of Bipolaris sorokiniana isolates using URP-PCR
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/99269 |
Resumo: | Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650- bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP’s are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana. |
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Mann, Michele BertoniSpadari, Cristina de CastroFeltrin, ThaisaFrazzon, Ana Paula GuedesGermani, Jose CarlosVan der Sand, Sueli Terezinha2014-08-08T02:07:05Z20141982-5676http://hdl.handle.net/10183/99269000919818Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650- bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP’s are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana.application/pdfengTropical Plant Pathology, Brasília. Vol. 39, no. 21 (Mar./Apr. 2014), p. 163-171FungosBipolaris sorokinianaReação em cadeia da polimeraseTrigoTriticum aestivunSpot blotch diseaseWheatGenetic variability of Bipolaris sorokiniana isolates using URP-PCRinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000919818.pdf000919818.pdfTexto completo (inglês)application/pdf2102548http://www.lume.ufrgs.br/bitstream/10183/99269/1/000919818.pdfd6a180952d87d47320f1fcf9e0ae289cMD51TEXT000919818.pdf.txt000919818.pdf.txtExtracted Texttext/plain29579http://www.lume.ufrgs.br/bitstream/10183/99269/2/000919818.pdf.txtb0dd40bb9c0672b1ce959bc269ab96e4MD52THUMBNAIL000919818.pdf.jpg000919818.pdf.jpgGenerated Thumbnailimage/jpeg2290http://www.lume.ufrgs.br/bitstream/10183/99269/3/000919818.pdf.jpg814a1b0a15ca8898f34beccd30a00813MD5310183/992692021-09-18 04:53:59.1808oai:www.lume.ufrgs.br:10183/99269Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2021-09-18T07:53:59Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
spellingShingle |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR Mann, Michele Bertoni Fungos Bipolaris sorokiniana Reação em cadeia da polimerase Trigo Triticum aestivun Spot blotch disease Wheat |
title_short |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_full |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_fullStr |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_full_unstemmed |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
title_sort |
Genetic variability of Bipolaris sorokiniana isolates using URP-PCR |
author |
Mann, Michele Bertoni |
author_facet |
Mann, Michele Bertoni Spadari, Cristina de Castro Feltrin, Thaisa Frazzon, Ana Paula Guedes Germani, Jose Carlos Van der Sand, Sueli Terezinha |
author_role |
author |
author2 |
Spadari, Cristina de Castro Feltrin, Thaisa Frazzon, Ana Paula Guedes Germani, Jose Carlos Van der Sand, Sueli Terezinha |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mann, Michele Bertoni Spadari, Cristina de Castro Feltrin, Thaisa Frazzon, Ana Paula Guedes Germani, Jose Carlos Van der Sand, Sueli Terezinha |
dc.subject.por.fl_str_mv |
Fungos Bipolaris sorokiniana Reação em cadeia da polimerase Trigo |
topic |
Fungos Bipolaris sorokiniana Reação em cadeia da polimerase Trigo Triticum aestivun Spot blotch disease Wheat |
dc.subject.eng.fl_str_mv |
Triticum aestivun Spot blotch disease Wheat |
description |
Spot blotch disease, caused by Bipolaris sorokiniana, is one of the major diseases of wheat and is responsible for large losses of wheat crops worldwide. We used polymerase chain reaction (PCR) with universal rice primers (URP) for molecular characterization of 60 monosporic B. sorokiniana isolates from Brazil and other countries, and evaluated the diversity of the samples. PCR amplification generated 232 different DNA fragments ranging in size from 100 to 2018 bp. The primers URP-4R, URP-2R, and URP-1F generated greater numbers of amplified fragments (36, 30, and 25, respectively) from the single-spore isolates, and some diversity was observed among the isolates generated using these primers. The primers URP-2F, URP-6R, URP-17R, URP-30F, and URP-38F produced a pattern of monomorphic fragments and 73% of the isolates showed an average of 44 different DNA-amplified fragments. Primer URP-2F generated a 578-bp fragment that was common to 83.7% of the isolates; primer URP-6R generated a 548-bp fragment and primer URP-38F generated a 650- bp product that was common to 89.1% and 80% of the isolates, respectively. The URP-PCR primers provided important information about the genetic profiles of the monosporic cultures, which showed intraspecific variability among the monoconidial isolates and among the monosporic cultures that originated from the same polysporic strain. Our results indicate that URP’s are sensitive and give reproducible results for assaying the genetic variability of B. sorokiniana. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-08-08T02:07:05Z |
dc.date.issued.fl_str_mv |
2014 |
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http://hdl.handle.net/10183/99269 |
dc.identifier.issn.pt_BR.fl_str_mv |
1982-5676 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000919818 |
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1982-5676 000919818 |
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http://hdl.handle.net/10183/99269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Tropical Plant Pathology, Brasília. Vol. 39, no. 21 (Mar./Apr. 2014), p. 163-171 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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