Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil

Detalhes bibliográficos
Autor(a) principal: Beserra Jr.,José Evando A.
Data de Publicação: 2011
Outros Autores: Carvalho,Murilo G. de, Barguil,Beatriz M., Zerbini,F. Murilo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008
Resumo: Senna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales).
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spelling Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in BrazilSenna virus XSenna virus Ymolecular characterizationSenna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales).Sociedade Brasileira de Fitopatologia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008Tropical Plant Pathology v.36 n.2 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000200008info:eu-repo/semantics/openAccessBeserra Jr.,José Evando A.Carvalho,Murilo G. deBarguil,Beatriz M.Zerbini,F. Muriloeng2011-06-17T00:00:00Zoai:scielo:S1982-56762011000200008Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-06-17T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
title Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
spellingShingle Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
Beserra Jr.,José Evando A.
Senna virus X
Senna virus Y
molecular characterization
title_short Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
title_full Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
title_fullStr Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
title_full_unstemmed Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
title_sort Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
author Beserra Jr.,José Evando A.
author_facet Beserra Jr.,José Evando A.
Carvalho,Murilo G. de
Barguil,Beatriz M.
Zerbini,F. Murilo
author_role author
author2 Carvalho,Murilo G. de
Barguil,Beatriz M.
Zerbini,F. Murilo
author2_role author
author
author
dc.contributor.author.fl_str_mv Beserra Jr.,José Evando A.
Carvalho,Murilo G. de
Barguil,Beatriz M.
Zerbini,F. Murilo
dc.subject.por.fl_str_mv Senna virus X
Senna virus Y
molecular characterization
topic Senna virus X
Senna virus Y
molecular characterization
description Senna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales).
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762011000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.36 n.2 2011
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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