Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008 |
Resumo: | Senna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales). |
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Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in BrazilSenna virus XSenna virus Ymolecular characterizationSenna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales).Sociedade Brasileira de Fitopatologia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008Tropical Plant Pathology v.36 n.2 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000200008info:eu-repo/semantics/openAccessBeserra Jr.,José Evando A.Carvalho,Murilo G. deBarguil,Beatriz M.Zerbini,F. Muriloeng2011-06-17T00:00:00Zoai:scielo:S1982-56762011000200008Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-06-17T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
title |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
spellingShingle |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil Beserra Jr.,José Evando A. Senna virus X Senna virus Y molecular characterization |
title_short |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
title_full |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
title_fullStr |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
title_full_unstemmed |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
title_sort |
Partial genome sequence of a Potyvirus and of a virus in the order Tymovirales found in Senna macranthera in Brazil |
author |
Beserra Jr.,José Evando A. |
author_facet |
Beserra Jr.,José Evando A. Carvalho,Murilo G. de Barguil,Beatriz M. Zerbini,F. Murilo |
author_role |
author |
author2 |
Carvalho,Murilo G. de Barguil,Beatriz M. Zerbini,F. Murilo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Beserra Jr.,José Evando A. Carvalho,Murilo G. de Barguil,Beatriz M. Zerbini,F. Murilo |
dc.subject.por.fl_str_mv |
Senna virus X Senna virus Y molecular characterization |
topic |
Senna virus X Senna virus Y molecular characterization |
description |
Senna macranthera is a common tropical tree native to South- and Northeastern Brazil. In July 2006, symptoms of chlorotic spots in the young leaves and mild mosaic in the old leaves were observed in S. macranthera trees at Viçosa, State of Minas Gerais, Brazil. Electron microscopy examination of leaf dip preparations from the young leaves revealed the presence of flexuous rods. Viral RNA was extracted from purified virion preparations and used as a template for the RT-PCR-based cloning of viral genomic fragments. Two fragments (0.9 kb and 2.0 kb in length) were cloned. Sequence analysis of the 0.9 kb fragment indicated that it corresponded to the NIb coding region of a potyvirus, with nucleotide (nt) identity level of 72% for Pepper mottle virus (PepMoV) and Peru tomato mosaic virus (PTV), while analysis of the 2.0 kb fragment indicated that it corresponded to the putative replicase ORF, which displayed 74% nt identity with the ORF1 (replicase) of Garlic virus A (GarV-A). Based on molecular analysis and the isolates' host range, it is concluded that the viral fragments represent new viruses, for which we propose the names Senna virus Y (genus Potyvirus, family Potyviridae) and Senna virus X (unassigned genus, order Tymovirales). |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762011000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.36 n.2 2011 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585422831616 |