Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus

Detalhes bibliográficos
Autor(a) principal: Fu,Rongtao
Data de Publicação: 2014
Outros Autores: Yin,Chuanchun, Deng,Qiming, Li,Ping, Zheng,Aiping
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300003
Resumo: Villosiclava virens (anamorph: Ustilaginoidea virens) was isolated from infected rice plants, where it caused false smut. Similar to other ascomycetes, the sexual compatibility of V. virens is controlled by the mating type (MAT) locus. In this study, we applied a PCR-based approach to screen mating type genes in the single asexual spore isolate. Our results showed that V. virens possesses the two master genes required for mating (MAT1-1-1 and MAT1-2-1), suggesting that this fungus is likely to be homothallic. Mating type primer pairs were developed to genotype the single ascospore isolates from different places in China. These analyses provided further evidence that V. virens is a homothallic species and suggest novel genetic mechanisms of sexual reproduction in V. virens.
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spelling Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungusUstilaginoidea virensasexual sporeshomothallicMATmultiplex-PCRVillosiclava virens (anamorph: Ustilaginoidea virens) was isolated from infected rice plants, where it caused false smut. Similar to other ascomycetes, the sexual compatibility of V. virens is controlled by the mating type (MAT) locus. In this study, we applied a PCR-based approach to screen mating type genes in the single asexual spore isolate. Our results showed that V. virens possesses the two master genes required for mating (MAT1-1-1 and MAT1-2-1), suggesting that this fungus is likely to be homothallic. Mating type primer pairs were developed to genotype the single ascospore isolates from different places in China. These analyses provided further evidence that V. virens is a homothallic species and suggest novel genetic mechanisms of sexual reproduction in V. virens.Sociedade Brasileira de Fitopatologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300003Tropical Plant Pathology v.39 n.3 2014reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762014000300003info:eu-repo/semantics/openAccessFu,RongtaoYin,ChuanchunDeng,QimingLi,PingZheng,Aipingeng2014-06-03T00:00:00Zoai:scielo:S1982-56762014000300003Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2014-06-03T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
title Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
spellingShingle Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
Fu,Rongtao
Ustilaginoidea virens
asexual spores
homothallic
MAT
multiplex-PCR
title_short Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
title_full Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
title_fullStr Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
title_full_unstemmed Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
title_sort Mating type loci analysis indicates that Villosiclava virens, the casual agent of false smut disease of rice, is a homothallic fungus
author Fu,Rongtao
author_facet Fu,Rongtao
Yin,Chuanchun
Deng,Qiming
Li,Ping
Zheng,Aiping
author_role author
author2 Yin,Chuanchun
Deng,Qiming
Li,Ping
Zheng,Aiping
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fu,Rongtao
Yin,Chuanchun
Deng,Qiming
Li,Ping
Zheng,Aiping
dc.subject.por.fl_str_mv Ustilaginoidea virens
asexual spores
homothallic
MAT
multiplex-PCR
topic Ustilaginoidea virens
asexual spores
homothallic
MAT
multiplex-PCR
description Villosiclava virens (anamorph: Ustilaginoidea virens) was isolated from infected rice plants, where it caused false smut. Similar to other ascomycetes, the sexual compatibility of V. virens is controlled by the mating type (MAT) locus. In this study, we applied a PCR-based approach to screen mating type genes in the single asexual spore isolate. Our results showed that V. virens possesses the two master genes required for mating (MAT1-1-1 and MAT1-2-1), suggesting that this fungus is likely to be homothallic. Mating type primer pairs were developed to genotype the single ascospore isolates from different places in China. These analyses provided further evidence that V. virens is a homothallic species and suggest novel genetic mechanisms of sexual reproduction in V. virens.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000300003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762014000300003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.39 n.3 2014
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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