Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule

Detalhes bibliográficos
Autor(a) principal: Jiménez,Nilda Z. A.
Data de Publicação: 2010
Outros Autores: Montano,Helena G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008
Resumo: Phytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA.
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spelling Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edulebitter melonperiwinklechayoteDNAwitches'-broomPhytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA.Sociedade Brasileira de Fitopatologia2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008Tropical Plant Pathology v.35 n.6 2010reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762010000600008info:eu-repo/semantics/openAccessJiménez,Nilda Z. A.Montano,Helena G.eng2011-03-02T00:00:00Zoai:scielo:S1982-56762010000600008Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-03-02T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
title Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
spellingShingle Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
Jiménez,Nilda Z. A.
bitter melon
periwinkle
chayote
DNA
witches'-broom
title_short Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
title_full Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
title_fullStr Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
title_full_unstemmed Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
title_sort Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
author Jiménez,Nilda Z. A.
author_facet Jiménez,Nilda Z. A.
Montano,Helena G.
author_role author
author2 Montano,Helena G.
author2_role author
dc.contributor.author.fl_str_mv Jiménez,Nilda Z. A.
Montano,Helena G.
dc.subject.por.fl_str_mv bitter melon
periwinkle
chayote
DNA
witches'-broom
topic bitter melon
periwinkle
chayote
DNA
witches'-broom
description Phytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762010000600008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.35 n.6 2010
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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