Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008 |
Resumo: | Phytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA. |
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Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edulebitter melonperiwinklechayoteDNAwitches'-broomPhytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA.Sociedade Brasileira de Fitopatologia2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008Tropical Plant Pathology v.35 n.6 2010reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762010000600008info:eu-repo/semantics/openAccessJiménez,Nilda Z. A.Montano,Helena G.eng2011-03-02T00:00:00Zoai:scielo:S1982-56762010000600008Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-03-02T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
title |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
spellingShingle |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule Jiménez,Nilda Z. A. bitter melon periwinkle chayote DNA witches'-broom |
title_short |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
title_full |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
title_fullStr |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
title_full_unstemmed |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
title_sort |
Detection of phytoplasma in desiccated tissue of Momordica charantia, Catharanthus roseus and Sechium edule |
author |
Jiménez,Nilda Z. A. |
author_facet |
Jiménez,Nilda Z. A. Montano,Helena G. |
author_role |
author |
author2 |
Montano,Helena G. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Jiménez,Nilda Z. A. Montano,Helena G. |
dc.subject.por.fl_str_mv |
bitter melon periwinkle chayote DNA witches'-broom |
topic |
bitter melon periwinkle chayote DNA witches'-broom |
description |
Phytoplasmas can cause yield losses in numerous crops worldwide. In Brazil, phytoplasmal diseases have been reported in plant species, distributed among 29 botanical families. Chayote witches'-broom (ChWB) is reported as one of the diseases of economic importance, associated with phytoplasma in the country, and bitter melon is a natural alternative host of the phytoplasma associated with the disease. Periwinkle is an ornamental plant that can naturally host phytoplasmas. An attempt to detect phytoplasma in desiccated leaf tissues collected from naturally diseased chayote, bitter melon and periwinkle plants was made. On the basis of phytoplasma-specific DNA amplification in PCR, phytoplasma was detected in desiccated chayote and bitter melon samples affected by witches'-broom, as well as in desiccated periwinkle exhibiting chlorosis and small leaves. DNA extraction was achieved using 0.25 g and 0.5 g of bitter melon and periwinkle dessicated tissue. These results encourage trials in utilizing dried plant tissues for the detection of phytoplasmas in other plant species, since dried samples are easy to preserve and transport, and yield good amounts of DNA. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762010000600008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.35 n.6 2010 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824585394520064 |