Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/56064 |
Resumo: | Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India. |
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Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, ÍndiaLittle LeafPeriwinklePhytoplasmaSEMWitches’ Broom.Agricultiral SciencesCathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.Cathranthus roseus também conhecida como pervinca, planta ornamental contém vários valores medicinais, foi encontrada com poucos sintomas de folhas e vassouras de bruxa no local de Shahjahanpur, com incidência de até 8%. A etiologia do fitoplasma foi confirmada através do exame de microscopia eletrônica de varredura. Análises moleculares adicionais por meio de PCR aninhado, análise filogênica e silco RFLP confirmaram a associação do fitoplasma do subgrupo 16SrIX-C em pequenas plantas de folhas e vassouras de bruxa, que é o primeiro relatório da Índia.EDUFU2022-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5606410.14393/BJ-v38n0a2022-56064Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38026Bioscience Journal ; v. 38 (2022): Continuous Publication; e380261981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/56064/33647India; ContemporaryCopyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwarihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTiwari, Niraj NathJain, Ravindra KumarAjay Kumar Tiwari2022-03-31T16:55:20Zoai:ojs.www.seer.ufu.br:article/56064Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-03-31T16:55:20Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
title |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
spellingShingle |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia Tiwari, Niraj Nath Little Leaf Periwinkle Phytoplasma SEM Witches’ Broom. Agricultiral Sciences |
title_short |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
title_full |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
title_fullStr |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
title_full_unstemmed |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
title_sort |
Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia |
author |
Tiwari, Niraj Nath |
author_facet |
Tiwari, Niraj Nath Jain, Ravindra Kumar Ajay Kumar Tiwari |
author_role |
author |
author2 |
Jain, Ravindra Kumar Ajay Kumar Tiwari |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Tiwari, Niraj Nath Jain, Ravindra Kumar Ajay Kumar Tiwari |
dc.subject.por.fl_str_mv |
Little Leaf Periwinkle Phytoplasma SEM Witches’ Broom. Agricultiral Sciences |
topic |
Little Leaf Periwinkle Phytoplasma SEM Witches’ Broom. Agricultiral Sciences |
description |
Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/56064 10.14393/BJ-v38n0a2022-56064 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/56064 |
identifier_str_mv |
10.14393/BJ-v38n0a2022-56064 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/56064/33647 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwari https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwari https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
India; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38026 Bioscience Journal ; v. 38 (2022): Continuous Publication; e38026 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
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1797069082929922048 |