Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia

Detalhes bibliográficos
Autor(a) principal: Tiwari, Niraj Nath
Data de Publicação: 2022
Outros Autores: Jain, Ravindra Kumar, Ajay Kumar Tiwari
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/56064
Resumo: Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.
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spelling Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, ÍndiaLittle LeafPeriwinklePhytoplasmaSEMWitches’ Broom.Agricultiral SciencesCathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.Cathranthus roseus também conhecida como pervinca, planta ornamental contém vários valores medicinais, foi encontrada com poucos sintomas de folhas e vassouras de bruxa no local de Shahjahanpur, com incidência de até 8%. A etiologia do fitoplasma foi confirmada através do exame de microscopia eletrônica de varredura. Análises moleculares adicionais por meio de PCR aninhado, análise filogênica e silco RFLP confirmaram a associação do fitoplasma do subgrupo 16SrIX-C em pequenas plantas de folhas e vassouras de bruxa, que é o primeiro relatório da Índia.EDUFU2022-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/5606410.14393/BJ-v38n0a2022-56064Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38026Bioscience Journal ; v. 38 (2022): Continuous Publication; e380261981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/56064/33647India; ContemporaryCopyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwarihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTiwari, Niraj NathJain, Ravindra KumarAjay Kumar Tiwari2022-03-31T16:55:20Zoai:ojs.www.seer.ufu.br:article/56064Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-03-31T16:55:20Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
title Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
spellingShingle Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
Tiwari, Niraj Nath
Little Leaf
Periwinkle
Phytoplasma
SEM
Witches’ Broom.
Agricultiral Sciences
title_short Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
title_full Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
title_fullStr Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
title_full_unstemmed Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
title_sort Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
author Tiwari, Niraj Nath
author_facet Tiwari, Niraj Nath
Jain, Ravindra Kumar
Ajay Kumar Tiwari
author_role author
author2 Jain, Ravindra Kumar
Ajay Kumar Tiwari
author2_role author
author
dc.contributor.author.fl_str_mv Tiwari, Niraj Nath
Jain, Ravindra Kumar
Ajay Kumar Tiwari
dc.subject.por.fl_str_mv Little Leaf
Periwinkle
Phytoplasma
SEM
Witches’ Broom.
Agricultiral Sciences
topic Little Leaf
Periwinkle
Phytoplasma
SEM
Witches’ Broom.
Agricultiral Sciences
description Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/56064
10.14393/BJ-v38n0a2022-56064
url https://seer.ufu.br/index.php/biosciencejournal/article/view/56064
identifier_str_mv 10.14393/BJ-v38n0a2022-56064
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/56064/33647
dc.rights.driver.fl_str_mv Copyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwari
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwari
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv India; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 38 (2022): Continuous Publication; e38026
Bioscience Journal ; v. 38 (2022): Continuous Publication; e38026
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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