Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil

Detalhes bibliográficos
Autor(a) principal: Aranha,Silvia de Araujo
Data de Publicação: 2011
Outros Autores: Albuquerque,Leonardo Cunha de, Boiteux,Leonardo Silva, Inoue-Nagata,Alice Kazuko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Tropical plant pathology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000100002
Resumo: A survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.
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spelling Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in BrazilSida micrantha mosaic virusgeminivirusSimMVA survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.Sociedade Brasileira de Fitopatologia2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000100002Tropical Plant Pathology v.36 n.1 2011reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762011000100002info:eu-repo/semantics/openAccessAranha,Silvia de AraujoAlbuquerque,Leonardo Cunha deBoiteux,Leonardo SilvaInoue-Nagata,Alice Kazukoeng2011-05-04T00:00:00Zoai:scielo:S1982-56762011000100002Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2011-05-04T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse
dc.title.none.fl_str_mv Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
spellingShingle Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
Aranha,Silvia de Araujo
Sida micrantha mosaic virus
geminivirus
SimMV
title_short Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_full Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_fullStr Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_full_unstemmed Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
title_sort Detection and complete genome characterization of a begomovirus infecting okra (Abelmoschus esculentus) in Brazil
author Aranha,Silvia de Araujo
author_facet Aranha,Silvia de Araujo
Albuquerque,Leonardo Cunha de
Boiteux,Leonardo Silva
Inoue-Nagata,Alice Kazuko
author_role author
author2 Albuquerque,Leonardo Cunha de
Boiteux,Leonardo Silva
Inoue-Nagata,Alice Kazuko
author2_role author
author
author
dc.contributor.author.fl_str_mv Aranha,Silvia de Araujo
Albuquerque,Leonardo Cunha de
Boiteux,Leonardo Silva
Inoue-Nagata,Alice Kazuko
dc.subject.por.fl_str_mv Sida micrantha mosaic virus
geminivirus
SimMV
topic Sida micrantha mosaic virus
geminivirus
SimMV
description A survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DNA-A and DNA-B of isolate #5157 were cloned and their nucleotide sequences exhibited typical characteristics of New World bipartite begomoviruses. The DNA-A sequence shared 95.6% nucleotide identity with an isolate of Sida micrantha mosaic virus from Brazil and thus identified as its okra strain. The clones derived from #5157 were infectious to okra, Sida santaremnensis and to a group of Solanaceae plants when inoculated by biolistics after circularization of the isolated insert, followed by rolling circle amplification.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000100002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1982-56762011000100002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Tropical Plant Pathology v.36 n.1 2011
reponame:Tropical plant pathology (Online)
instname:Sociedade Brasileira de Fitopatologia
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia
instacron_str SBF
institution SBF
reponame_str Tropical plant pathology (Online)
collection Tropical plant pathology (Online)
repository.name.fl_str_mv Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia
repository.mail.fl_str_mv sbf-revista@ufla.br
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