Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034 |
Resumo: | ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants. |
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Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivoStevia rebaudianaAdventitious root culturesMorpho-anatomicalChemical profileABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.Sociedade Brasileira de Farmacognosia2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034Revista Brasileira de Farmacognosia v.27 n.1 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2016.08.007info:eu-repo/semantics/openAccessReis,Rafael V.Chierrito,Talita P.C.Silva,Thaila F.O.Albiero,Adriana L.M.Souza,Luiz A.Gonçalves,José E.Oliveira,Arildo J.B.Gonçalves,Regina A.C.eng2017-01-10T00:00:00Zoai:scielo:S0102-695X2017000100034Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2017-01-10T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
title |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
spellingShingle |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo Reis,Rafael V. Stevia rebaudiana Adventitious root cultures Morpho-anatomical Chemical profile |
title_short |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
title_full |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
title_fullStr |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
title_full_unstemmed |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
title_sort |
Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo |
author |
Reis,Rafael V. |
author_facet |
Reis,Rafael V. Chierrito,Talita P.C. Silva,Thaila F.O. Albiero,Adriana L.M. Souza,Luiz A. Gonçalves,José E. Oliveira,Arildo J.B. Gonçalves,Regina A.C. |
author_role |
author |
author2 |
Chierrito,Talita P.C. Silva,Thaila F.O. Albiero,Adriana L.M. Souza,Luiz A. Gonçalves,José E. Oliveira,Arildo J.B. Gonçalves,Regina A.C. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Reis,Rafael V. Chierrito,Talita P.C. Silva,Thaila F.O. Albiero,Adriana L.M. Souza,Luiz A. Gonçalves,José E. Oliveira,Arildo J.B. Gonçalves,Regina A.C. |
dc.subject.por.fl_str_mv |
Stevia rebaudiana Adventitious root cultures Morpho-anatomical Chemical profile |
topic |
Stevia rebaudiana Adventitious root cultures Morpho-anatomical Chemical profile |
description |
ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2016.08.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.27 n.1 2017 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122470143361024 |