Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo

Detalhes bibliográficos
Autor(a) principal: Reis,Rafael V.
Data de Publicação: 2017
Outros Autores: Chierrito,Talita P.C., Silva,Thaila F.O., Albiero,Adriana L.M., Souza,Luiz A., Gonçalves,José E., Oliveira,Arildo J.B., Gonçalves,Regina A.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034
Resumo: ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.
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spelling Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivoStevia rebaudianaAdventitious root culturesMorpho-anatomicalChemical profileABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.Sociedade Brasileira de Farmacognosia2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034Revista Brasileira de Farmacognosia v.27 n.1 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2016.08.007info:eu-repo/semantics/openAccessReis,Rafael V.Chierrito,Talita P.C.Silva,Thaila F.O.Albiero,Adriana L.M.Souza,Luiz A.Gonçalves,José E.Oliveira,Arildo J.B.Gonçalves,Regina A.C.eng2017-01-10T00:00:00Zoai:scielo:S0102-695X2017000100034Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2017-01-10T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
title Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
spellingShingle Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
Reis,Rafael V.
Stevia rebaudiana
Adventitious root cultures
Morpho-anatomical
Chemical profile
title_short Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
title_full Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
title_fullStr Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
title_full_unstemmed Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
title_sort Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo
author Reis,Rafael V.
author_facet Reis,Rafael V.
Chierrito,Talita P.C.
Silva,Thaila F.O.
Albiero,Adriana L.M.
Souza,Luiz A.
Gonçalves,José E.
Oliveira,Arildo J.B.
Gonçalves,Regina A.C.
author_role author
author2 Chierrito,Talita P.C.
Silva,Thaila F.O.
Albiero,Adriana L.M.
Souza,Luiz A.
Gonçalves,José E.
Oliveira,Arildo J.B.
Gonçalves,Regina A.C.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reis,Rafael V.
Chierrito,Talita P.C.
Silva,Thaila F.O.
Albiero,Adriana L.M.
Souza,Luiz A.
Gonçalves,José E.
Oliveira,Arildo J.B.
Gonçalves,Regina A.C.
dc.subject.por.fl_str_mv Stevia rebaudiana
Adventitious root cultures
Morpho-anatomical
Chemical profile
topic Stevia rebaudiana
Adventitious root cultures
Morpho-anatomical
Chemical profile
description ABSTRACT Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC–MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000100034
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2016.08.007
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.27 n.1 2017
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
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collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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