Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling

Detalhes bibliográficos
Autor(a) principal: Moradi-Afrapoli,Fahimeh
Data de Publicação: 2018
Outros Autores: Shokrzadeh,Mohammad, Barzegar,Fatemeh, Gorji-Bahri,Gilar, Zadali,Reza, Nejad Ebrahimi,Samad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027
Resumo: ABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity.
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spelling Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profilingDiterpeneCancer cell linesMTTCytotoxicityMolecular modelingABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity.Sociedade Brasileira de Farmacognosia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027Revista Brasileira de Farmacognosia v.28 n.1 2018reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2017.11.007info:eu-repo/semantics/openAccessMoradi-Afrapoli,FahimehShokrzadeh,MohammadBarzegar,FatemehGorji-Bahri,GilarZadali,RezaNejad Ebrahimi,Samadeng2018-03-20T00:00:00Zoai:scielo:S0102-695X2018000100027Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2018-03-20T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
title Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
spellingShingle Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
Moradi-Afrapoli,Fahimeh
Diterpene
Cancer cell lines
MTT
Cytotoxicity
Molecular modeling
title_short Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
title_full Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
title_fullStr Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
title_full_unstemmed Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
title_sort Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
author Moradi-Afrapoli,Fahimeh
author_facet Moradi-Afrapoli,Fahimeh
Shokrzadeh,Mohammad
Barzegar,Fatemeh
Gorji-Bahri,Gilar
Zadali,Reza
Nejad Ebrahimi,Samad
author_role author
author2 Shokrzadeh,Mohammad
Barzegar,Fatemeh
Gorji-Bahri,Gilar
Zadali,Reza
Nejad Ebrahimi,Samad
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moradi-Afrapoli,Fahimeh
Shokrzadeh,Mohammad
Barzegar,Fatemeh
Gorji-Bahri,Gilar
Zadali,Reza
Nejad Ebrahimi,Samad
dc.subject.por.fl_str_mv Diterpene
Cancer cell lines
MTT
Cytotoxicity
Molecular modeling
topic Diterpene
Cancer cell lines
MTT
Cytotoxicity
Molecular modeling
description ABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2017.11.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.28 n.1 2018
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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