Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027 |
Resumo: | ABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity. |
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Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profilingDiterpeneCancer cell linesMTTCytotoxicityMolecular modelingABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity.Sociedade Brasileira de Farmacognosia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027Revista Brasileira de Farmacognosia v.28 n.1 2018reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2017.11.007info:eu-repo/semantics/openAccessMoradi-Afrapoli,FahimehShokrzadeh,MohammadBarzegar,FatemehGorji-Bahri,GilarZadali,RezaNejad Ebrahimi,Samadeng2018-03-20T00:00:00Zoai:scielo:S0102-695X2018000100027Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2018-03-20T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
title |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
spellingShingle |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling Moradi-Afrapoli,Fahimeh Diterpene Cancer cell lines MTT Cytotoxicity Molecular modeling |
title_short |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
title_full |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
title_fullStr |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
title_full_unstemmed |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
title_sort |
Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling |
author |
Moradi-Afrapoli,Fahimeh |
author_facet |
Moradi-Afrapoli,Fahimeh Shokrzadeh,Mohammad Barzegar,Fatemeh Gorji-Bahri,Gilar Zadali,Reza Nejad Ebrahimi,Samad |
author_role |
author |
author2 |
Shokrzadeh,Mohammad Barzegar,Fatemeh Gorji-Bahri,Gilar Zadali,Reza Nejad Ebrahimi,Samad |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moradi-Afrapoli,Fahimeh Shokrzadeh,Mohammad Barzegar,Fatemeh Gorji-Bahri,Gilar Zadali,Reza Nejad Ebrahimi,Samad |
dc.subject.por.fl_str_mv |
Diterpene Cancer cell lines MTT Cytotoxicity Molecular modeling |
topic |
Diterpene Cancer cell lines MTT Cytotoxicity Molecular modeling |
description |
ABSTRACT Screening of medicinal plants from Iranian flora against human cancer cell-lines have shown that an hexane extract from roots of Salvia sahendica Boiss. & Buhse, Lamiaceae, is active against human cervical cancer (HeLa) and colorectal adenocarcinoma (Caco-2) cell-lines at the test concentration of 100 µg/ml (100% inhibition). Cytotoxicity of the extract was localized with the aid of HPLC-time-based activity profiling adapted to the tetrazolium colorimetric bioassay. Four abietane-type diterpenoids in active time-windows were identified as cytotoxic compounds namely: sahandone (1), sahandol (2), 12-deoxy-salvipisone (3) and sahandinone (4). Compound 1 showed the highest toxicity against HeLa cells (IC50 = 5.6 ± 0.1 µg/ml), which was comparable with betulinic acid (IC50 = 4.3 ± 1.2 µg/ml), the positive control. Compound 2 was active against the HeLa cells (IC50 = 8.9 ± 0.7 µg/ml) but not the Caco-2 cell-line. Compounds 1, 3 and 4 exhibited moderate activity (IC50 = 22.9–41.4 µg/ml) against the Caco-2 cells. This study reveals that the HeLa cells are more sensitive to all tested compounds than the Caco-2 cells. In silico molecular docking study showed a rigid binding of the compounds to tyrosine kinase pp60src, and proved their cytotoxic activity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000100027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2017.11.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.28 n.1 2018 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122470613123072 |