Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Rodney Alexandre Ferreira
Data de Publicação: 2011
Outros Autores: Carvalho,João Ernesto de, Sousa,Ilza Maria de Oliveira, Antônio,Marcia Aparecida, Pizão,Paulo Eduardo, Kohn,Luciana Konecny, Amaral,Maria do Carmo Estanislau do, Bittrich,Volker, Foglio,Mary Ann
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400010
Resumo: Gaylussacia brasiliensis (Spreng.) Meissn., Ericaceae, is used in folk medicine for treatment of several inflammatory processes and as healing agent. The scope of this work was to evaluate the in vitro antiproliferative activity of crude dichloromethane extract (CHD) and to identify the compound(s) responsible for this activity. CHD was evaluated and showed a concentration dependent inhibition on all cells lines. Therefore CHD was submitted to several classical columns chromatography providing the most active fraction (FC), inhibiting all cells line at 25 µg/mL. FC was further fractionated affording isolated compound 2β, 3β-dihydroxy-urs-12-ene-28-oic acid , identified on basis of 2D-NMR experiments and showed concentration-dependent activity and selectivity for kidney and breast cell lines.
id SBFGNOSIA-1_1cec46a2c157cdc132e16dfa473c4651
oai_identifier_str oai:scielo:S0102-695X2011000400010
network_acronym_str SBFGNOSIA-1
network_name_str Revista Brasileira de Farmacognosia (Online)
repository_id_str
spelling Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensisantiproliferativeGaylussacia brasiliensismedicinal plantsGaylussacia brasiliensis (Spreng.) Meissn., Ericaceae, is used in folk medicine for treatment of several inflammatory processes and as healing agent. The scope of this work was to evaluate the in vitro antiproliferative activity of crude dichloromethane extract (CHD) and to identify the compound(s) responsible for this activity. CHD was evaluated and showed a concentration dependent inhibition on all cells lines. Therefore CHD was submitted to several classical columns chromatography providing the most active fraction (FC), inhibiting all cells line at 25 µg/mL. FC was further fractionated affording isolated compound 2β, 3β-dihydroxy-urs-12-ene-28-oic acid , identified on basis of 2D-NMR experiments and showed concentration-dependent activity and selectivity for kidney and breast cell lines.Sociedade Brasileira de Farmacognosia2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400010Revista Brasileira de Farmacognosia v.21 n.4 2011reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2011005000116info:eu-repo/semantics/openAccessRodrigues,Rodney Alexandre FerreiraCarvalho,João Ernesto deSousa,Ilza Maria de OliveiraAntônio,Marcia AparecidaPizão,Paulo EduardoKohn,Luciana KonecnyAmaral,Maria do Carmo Estanislau doBittrich,VolkerFoglio,Mary Anneng2011-08-15T00:00:00Zoai:scielo:S0102-695X2011000400010Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2011-08-15T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
title Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
spellingShingle Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
Rodrigues,Rodney Alexandre Ferreira
antiproliferative
Gaylussacia brasiliensis
medicinal plants
title_short Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
title_full Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
title_fullStr Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
title_full_unstemmed Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
title_sort Antiproliferative activity, isolation and identification of active compound from Gaylussacia brasiliensis
author Rodrigues,Rodney Alexandre Ferreira
author_facet Rodrigues,Rodney Alexandre Ferreira
Carvalho,João Ernesto de
Sousa,Ilza Maria de Oliveira
Antônio,Marcia Aparecida
Pizão,Paulo Eduardo
Kohn,Luciana Konecny
Amaral,Maria do Carmo Estanislau do
Bittrich,Volker
Foglio,Mary Ann
author_role author
author2 Carvalho,João Ernesto de
Sousa,Ilza Maria de Oliveira
Antônio,Marcia Aparecida
Pizão,Paulo Eduardo
Kohn,Luciana Konecny
Amaral,Maria do Carmo Estanislau do
Bittrich,Volker
Foglio,Mary Ann
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues,Rodney Alexandre Ferreira
Carvalho,João Ernesto de
Sousa,Ilza Maria de Oliveira
Antônio,Marcia Aparecida
Pizão,Paulo Eduardo
Kohn,Luciana Konecny
Amaral,Maria do Carmo Estanislau do
Bittrich,Volker
Foglio,Mary Ann
dc.subject.por.fl_str_mv antiproliferative
Gaylussacia brasiliensis
medicinal plants
topic antiproliferative
Gaylussacia brasiliensis
medicinal plants
description Gaylussacia brasiliensis (Spreng.) Meissn., Ericaceae, is used in folk medicine for treatment of several inflammatory processes and as healing agent. The scope of this work was to evaluate the in vitro antiproliferative activity of crude dichloromethane extract (CHD) and to identify the compound(s) responsible for this activity. CHD was evaluated and showed a concentration dependent inhibition on all cells lines. Therefore CHD was submitted to several classical columns chromatography providing the most active fraction (FC), inhibiting all cells line at 25 µg/mL. FC was further fractionated affording isolated compound 2β, 3β-dihydroxy-urs-12-ene-28-oic acid , identified on basis of 2D-NMR experiments and showed concentration-dependent activity and selectivity for kidney and breast cell lines.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2011005000116
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.21 n.4 2011
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
_version_ 1752122466091663360

Registros relacionados