Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

Detalhes bibliográficos
Autor(a) principal: Batista,Ronan
Data de Publicação: 2009
Outros Autores: Brandão,Geraldo C., Braga,Fernão C., Oliveira,Alaíde B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000100009
Resumo: Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC50 = 140.6 μg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC50 = 980 μg/mL), DF (LC50 = 140.6 μg/mL), 1 (LC50 = 15.9 μg/mL) and 2 (LC50 = 29.8 μg/mL) were found to be cytotoxic, while the water fraction (WF, LC50 >> 1000 μg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.
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spelling Cytotoxicity of Wedelia paludosa D.C. extracts and constituentsWedelia paludosaAsteraceaecytotoxic activitybrine shrimp lethality bioassaykaurenoic acidgrandiflorenic acidHPLCWedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC50 = 140.6 μg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC50 = 980 μg/mL), DF (LC50 = 140.6 μg/mL), 1 (LC50 = 15.9 μg/mL) and 2 (LC50 = 29.8 μg/mL) were found to be cytotoxic, while the water fraction (WF, LC50 >> 1000 μg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.Sociedade Brasileira de Farmacognosia2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000100009Revista Brasileira de Farmacognosia v.19 n.1a 2009reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2009000100009info:eu-repo/semantics/openAccessBatista,RonanBrandão,Geraldo C.Braga,Fernão C.Oliveira,Alaíde B.eng2009-08-11T00:00:00Zoai:scielo:S0102-695X2009000100009Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2009-08-11T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
title Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
spellingShingle Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
Batista,Ronan
Wedelia paludosa
Asteraceae
cytotoxic activity
brine shrimp lethality bioassay
kaurenoic acid
grandiflorenic acid
HPLC
title_short Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
title_full Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
title_fullStr Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
title_full_unstemmed Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
title_sort Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
author Batista,Ronan
author_facet Batista,Ronan
Brandão,Geraldo C.
Braga,Fernão C.
Oliveira,Alaíde B.
author_role author
author2 Brandão,Geraldo C.
Braga,Fernão C.
Oliveira,Alaíde B.
author2_role author
author
author
dc.contributor.author.fl_str_mv Batista,Ronan
Brandão,Geraldo C.
Braga,Fernão C.
Oliveira,Alaíde B.
dc.subject.por.fl_str_mv Wedelia paludosa
Asteraceae
cytotoxic activity
brine shrimp lethality bioassay
kaurenoic acid
grandiflorenic acid
HPLC
topic Wedelia paludosa
Asteraceae
cytotoxic activity
brine shrimp lethality bioassay
kaurenoic acid
grandiflorenic acid
HPLC
description Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC50 = 140.6 μg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC50 = 980 μg/mL), DF (LC50 = 140.6 μg/mL), 1 (LC50 = 15.9 μg/mL) and 2 (LC50 = 29.8 μg/mL) were found to be cytotoxic, while the water fraction (WF, LC50 >> 1000 μg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000100009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2009000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2009000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.19 n.1a 2009
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
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instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
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reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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