Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii

Detalhes bibliográficos
Autor(a) principal: Nascimento,Fabiana G.
Data de Publicação: 2014
Outros Autores: Faqueti,Amanda, Wilhelm,Jessica F., Wittkowski,Carolina, Tomczak,Folvi D., Borges,Sheila L., Yunes,Rosendo A., Franchi Jr.,Gilberto C., Nowill,Alexandre E., Cechinel Filho,Valdir, Machado,Marina da S., Freitas,Rilton A. de, Malheiros,Angela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545
Resumo: The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.
id SBFGNOSIA-1_26a66f1a8ab3bb202d8010b01644febb
oai_identifier_str oai:scielo:S0102-695X2014000500545
network_acronym_str SBFGNOSIA-1
network_name_str Revista Brasileira de Farmacognosia (Online)
repository_id_str
spelling Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottiiAllamandaChromatographyIridoidsK562 and Nalm6 cellsPlumericinThe aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.Sociedade Brasileira de Farmacognosia2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545Revista Brasileira de Farmacognosia v.24 n.5 2014reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2014.08.005info:eu-repo/semantics/openAccessNascimento,Fabiana G.Faqueti,AmandaWilhelm,Jessica F.Wittkowski,CarolinaTomczak,Folvi D.Borges,Sheila L.Yunes,Rosendo A.Franchi Jr.,Gilberto C.Nowill,Alexandre E.Cechinel Filho,ValdirMachado,Marina da S.Freitas,Rilton A. deMalheiros,Angelaeng2015-10-09T00:00:00Zoai:scielo:S0102-695X2014000500545Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-10-09T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
title Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
spellingShingle Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
Nascimento,Fabiana G.
Allamanda
Chromatography
Iridoids
K562 and Nalm6 cells
Plumericin
title_short Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
title_full Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
title_fullStr Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
title_full_unstemmed Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
title_sort Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
author Nascimento,Fabiana G.
author_facet Nascimento,Fabiana G.
Faqueti,Amanda
Wilhelm,Jessica F.
Wittkowski,Carolina
Tomczak,Folvi D.
Borges,Sheila L.
Yunes,Rosendo A.
Franchi Jr.,Gilberto C.
Nowill,Alexandre E.
Cechinel Filho,Valdir
Machado,Marina da S.
Freitas,Rilton A. de
Malheiros,Angela
author_role author
author2 Faqueti,Amanda
Wilhelm,Jessica F.
Wittkowski,Carolina
Tomczak,Folvi D.
Borges,Sheila L.
Yunes,Rosendo A.
Franchi Jr.,Gilberto C.
Nowill,Alexandre E.
Cechinel Filho,Valdir
Machado,Marina da S.
Freitas,Rilton A. de
Malheiros,Angela
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento,Fabiana G.
Faqueti,Amanda
Wilhelm,Jessica F.
Wittkowski,Carolina
Tomczak,Folvi D.
Borges,Sheila L.
Yunes,Rosendo A.
Franchi Jr.,Gilberto C.
Nowill,Alexandre E.
Cechinel Filho,Valdir
Machado,Marina da S.
Freitas,Rilton A. de
Malheiros,Angela
dc.subject.por.fl_str_mv Allamanda
Chromatography
Iridoids
K562 and Nalm6 cells
Plumericin
topic Allamanda
Chromatography
Iridoids
K562 and Nalm6 cells
Plumericin
description The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2014.08.005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.24 n.5 2014
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
_version_ 1752122469165039616