Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545 |
Resumo: | The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified. |
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Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottiiAllamandaChromatographyIridoidsK562 and Nalm6 cellsPlumericinThe aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.Sociedade Brasileira de Farmacognosia2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545Revista Brasileira de Farmacognosia v.24 n.5 2014reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2014.08.005info:eu-repo/semantics/openAccessNascimento,Fabiana G.Faqueti,AmandaWilhelm,Jessica F.Wittkowski,CarolinaTomczak,Folvi D.Borges,Sheila L.Yunes,Rosendo A.Franchi Jr.,Gilberto C.Nowill,Alexandre E.Cechinel Filho,ValdirMachado,Marina da S.Freitas,Rilton A. deMalheiros,Angelaeng2015-10-09T00:00:00Zoai:scielo:S0102-695X2014000500545Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-10-09T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
title |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
spellingShingle |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii Nascimento,Fabiana G. Allamanda Chromatography Iridoids K562 and Nalm6 cells Plumericin |
title_short |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
title_full |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
title_fullStr |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
title_full_unstemmed |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
title_sort |
Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii |
author |
Nascimento,Fabiana G. |
author_facet |
Nascimento,Fabiana G. Faqueti,Amanda Wilhelm,Jessica F. Wittkowski,Carolina Tomczak,Folvi D. Borges,Sheila L. Yunes,Rosendo A. Franchi Jr.,Gilberto C. Nowill,Alexandre E. Cechinel Filho,Valdir Machado,Marina da S. Freitas,Rilton A. de Malheiros,Angela |
author_role |
author |
author2 |
Faqueti,Amanda Wilhelm,Jessica F. Wittkowski,Carolina Tomczak,Folvi D. Borges,Sheila L. Yunes,Rosendo A. Franchi Jr.,Gilberto C. Nowill,Alexandre E. Cechinel Filho,Valdir Machado,Marina da S. Freitas,Rilton A. de Malheiros,Angela |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento,Fabiana G. Faqueti,Amanda Wilhelm,Jessica F. Wittkowski,Carolina Tomczak,Folvi D. Borges,Sheila L. Yunes,Rosendo A. Franchi Jr.,Gilberto C. Nowill,Alexandre E. Cechinel Filho,Valdir Machado,Marina da S. Freitas,Rilton A. de Malheiros,Angela |
dc.subject.por.fl_str_mv |
Allamanda Chromatography Iridoids K562 and Nalm6 cells Plumericin |
topic |
Allamanda Chromatography Iridoids K562 and Nalm6 cells Plumericin |
description |
The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The results clearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor compounds not yet identified. The seasonal influence on the production of secondary metabolites was verified. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000500545 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2014.08.005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.24 n.5 2014 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
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1752122469165039616 |