Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000100051 |
Resumo: | Mutagenic and cytotoxic effects of roots, stems and leaves of Limonium globuliferum Kuntze, Plumbaginaceae, aqueous extracts were studied by Allium, Ames, and MTT tests. These are plant, bacterial and mammalian cell assays, respectively. The Allium test analyses showed that aqueous extracts of this species have dose-dependent toxicity and induce chromosomal anomalies based on defects in the spindle fibers. EC50 values of root stem and leaf aqueous extracts were 32.5, 50, and 50 g/l, respectively. It was observed that there was an inverse correlation between root growth and extract concentration. The lowest mitotic index value (22.72 %) was found in L. globuliferum root extract. As a result of the chromosome aberrations test, sticky chromosomes, anaphase bridges, laggard chromosomes, and anaphase-telophase disorders were highly detected especially in high concentration of the extract. In the Ames test, mutagenic effects were determined at all concentrations of stem and leaf aqueous extracts and only two concentrations of root extracts of L. globuliferum. Most of the extracts induced cytotoxic effects by the MTT test based on mitochondrial activity. Nevertheless, some of the extracts induced t cell proliferation. |
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Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT testsAllium testAmes testLimonium globuliferumMTT testMutagenic and cytotoxic effects of roots, stems and leaves of Limonium globuliferum Kuntze, Plumbaginaceae, aqueous extracts were studied by Allium, Ames, and MTT tests. These are plant, bacterial and mammalian cell assays, respectively. The Allium test analyses showed that aqueous extracts of this species have dose-dependent toxicity and induce chromosomal anomalies based on defects in the spindle fibers. EC50 values of root stem and leaf aqueous extracts were 32.5, 50, and 50 g/l, respectively. It was observed that there was an inverse correlation between root growth and extract concentration. The lowest mitotic index value (22.72 %) was found in L. globuliferum root extract. As a result of the chromosome aberrations test, sticky chromosomes, anaphase bridges, laggard chromosomes, and anaphase-telophase disorders were highly detected especially in high concentration of the extract. In the Ames test, mutagenic effects were determined at all concentrations of stem and leaf aqueous extracts and only two concentrations of root extracts of L. globuliferum. Most of the extracts induced cytotoxic effects by the MTT test based on mitochondrial activity. Nevertheless, some of the extracts induced t cell proliferation.Sociedade Brasileira de Farmacognosia2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000100051Revista Brasileira de Farmacognosia v.24 n.1 2014reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/0102-695X20142413322info:eu-repo/semantics/openAccessEren,YasinÖzata,Ahmeteng2015-08-27T00:00:00Zoai:scielo:S0102-695X2014000100051Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2015-08-27T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
title |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
spellingShingle |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests Eren,Yasin Allium test Ames test Limonium globuliferum MTT test |
title_short |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
title_full |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
title_fullStr |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
title_full_unstemmed |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
title_sort |
Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests |
author |
Eren,Yasin |
author_facet |
Eren,Yasin Özata,Ahmet |
author_role |
author |
author2 |
Özata,Ahmet |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Eren,Yasin Özata,Ahmet |
dc.subject.por.fl_str_mv |
Allium test Ames test Limonium globuliferum MTT test |
topic |
Allium test Ames test Limonium globuliferum MTT test |
description |
Mutagenic and cytotoxic effects of roots, stems and leaves of Limonium globuliferum Kuntze, Plumbaginaceae, aqueous extracts were studied by Allium, Ames, and MTT tests. These are plant, bacterial and mammalian cell assays, respectively. The Allium test analyses showed that aqueous extracts of this species have dose-dependent toxicity and induce chromosomal anomalies based on defects in the spindle fibers. EC50 values of root stem and leaf aqueous extracts were 32.5, 50, and 50 g/l, respectively. It was observed that there was an inverse correlation between root growth and extract concentration. The lowest mitotic index value (22.72 %) was found in L. globuliferum root extract. As a result of the chromosome aberrations test, sticky chromosomes, anaphase bridges, laggard chromosomes, and anaphase-telophase disorders were highly detected especially in high concentration of the extract. In the Ames test, mutagenic effects were determined at all concentrations of stem and leaf aqueous extracts and only two concentrations of root extracts of L. globuliferum. Most of the extracts induced cytotoxic effects by the MTT test based on mitochondrial activity. Nevertheless, some of the extracts induced t cell proliferation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000100051 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2014000100051 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0102-695X20142413322 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.24 n.1 2014 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122468816912384 |