Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules17055255 http://hdl.handle.net/11449/26323 |
Resumo: | The mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin were mutagenic in the TA98 strain and kaempferol showed signs of mutagenicity in the other strains. The absence of hydroxyl groups, as in flavone, only signs of mutagenicity were shown in strain TA102, after metabolization and, among monohydroxylated flavones (3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone), the presence of hydroxyl groups only resulted in minor changes. Luteolin and fisetin also showed signs of mutagenicity in strain TA102. Finally, chrysin, which has only two hydroxy groups, at the 5-OH and 7-OH positions, also did not induce mutagenic activity in any of the bacterial strains used, under either activation condition. All the flavonoids were tested at concentrations varying from 2.6 to 30.7 nmol/plate for galangin and 12.1 to 225.0 nmol/plate for other flavonoids. In light of the above, it is necessary to clarify the conditions and the mechanisms that mediate the biological effects of flavonoids before treating them as therapeutical agents, since some compounds can be biotransformed into more genotoxic products; as is the case for galangin, kaempferol and quercetin. |
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Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) TestmutagenicityAmes testflavonoidsThe mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin were mutagenic in the TA98 strain and kaempferol showed signs of mutagenicity in the other strains. The absence of hydroxyl groups, as in flavone, only signs of mutagenicity were shown in strain TA102, after metabolization and, among monohydroxylated flavones (3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone), the presence of hydroxyl groups only resulted in minor changes. Luteolin and fisetin also showed signs of mutagenicity in strain TA102. Finally, chrysin, which has only two hydroxy groups, at the 5-OH and 7-OH positions, also did not induce mutagenic activity in any of the bacterial strains used, under either activation condition. All the flavonoids were tested at concentrations varying from 2.6 to 30.7 nmol/plate for galangin and 12.1 to 225.0 nmol/plate for other flavonoids. In light of the above, it is necessary to clarify the conditions and the mechanisms that mediate the biological effects of flavonoids before treating them as therapeutical agents, since some compounds can be biotransformed into more genotoxic products; as is the case for galangin, kaempferol and quercetin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP São Paulo State Univ, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, BR-14801902 Araraquara, SP, BrazilUNESP São Paulo State Univ, BR-11350000 Sao Vicente, SP, BrazilUNESP São Paulo State Univ, Dept Organ Chem, Inst Chem, BR-14800900 Araraquara, SP, BrazilUNESP São Paulo State Univ, Dept Biol Sci, Fac Pharmaceut Sci Araraquara, BR-14801902 Araraquara, SP, BrazilUNESP São Paulo State Univ, BR-11350000 Sao Vicente, SP, BrazilUNESP São Paulo State Univ, Dept Organ Chem, Inst Chem, BR-14800900 Araraquara, SP, BrazilMdpi AgUniversidade Estadual Paulista (Unesp)Resende, Flavia Aparecida [UNESP]Vilegas, Wagner [UNESP]Santos, Lourdes Campaner dos [UNESP]Varanda, Eliana Aparecida [UNESP]2014-05-20T14:21:08Z2014-05-20T14:21:08Z2012-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5255-5268application/pdfhttp://dx.doi.org/10.3390/molecules17055255Molecules. Basel: Mdpi Ag, v. 17, n. 5, p. 5255-5268, 2012.1420-3049http://hdl.handle.net/11449/2632310.3390/molecules17055255WOS:000304587600040WOS000304587600040.pdf792787722432683735382536406029770000-0003-3032-2556Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules3.0980,855info:eu-repo/semantics/openAccess2023-12-07T06:19:53Zoai:repositorio.unesp.br:11449/26323Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-07T06:19:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
title |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
spellingShingle |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test Resende, Flavia Aparecida [UNESP] mutagenicity Ames test flavonoids |
title_short |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
title_full |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
title_fullStr |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
title_full_unstemmed |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
title_sort |
Mutagenicity of Flavonoids Assayed by Bacterial Reverse Mutation (Ames) Test |
author |
Resende, Flavia Aparecida [UNESP] |
author_facet |
Resende, Flavia Aparecida [UNESP] Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] Varanda, Eliana Aparecida [UNESP] |
author_role |
author |
author2 |
Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] Varanda, Eliana Aparecida [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Resende, Flavia Aparecida [UNESP] Vilegas, Wagner [UNESP] Santos, Lourdes Campaner dos [UNESP] Varanda, Eliana Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
mutagenicity Ames test flavonoids |
topic |
mutagenicity Ames test flavonoids |
description |
The mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin were mutagenic in the TA98 strain and kaempferol showed signs of mutagenicity in the other strains. The absence of hydroxyl groups, as in flavone, only signs of mutagenicity were shown in strain TA102, after metabolization and, among monohydroxylated flavones (3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone), the presence of hydroxyl groups only resulted in minor changes. Luteolin and fisetin also showed signs of mutagenicity in strain TA102. Finally, chrysin, which has only two hydroxy groups, at the 5-OH and 7-OH positions, also did not induce mutagenic activity in any of the bacterial strains used, under either activation condition. All the flavonoids were tested at concentrations varying from 2.6 to 30.7 nmol/plate for galangin and 12.1 to 225.0 nmol/plate for other flavonoids. In light of the above, it is necessary to clarify the conditions and the mechanisms that mediate the biological effects of flavonoids before treating them as therapeutical agents, since some compounds can be biotransformed into more genotoxic products; as is the case for galangin, kaempferol and quercetin. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-01 2014-05-20T14:21:08Z 2014-05-20T14:21:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules17055255 Molecules. Basel: Mdpi Ag, v. 17, n. 5, p. 5255-5268, 2012. 1420-3049 http://hdl.handle.net/11449/26323 10.3390/molecules17055255 WOS:000304587600040 WOS000304587600040.pdf 7927877224326837 3538253640602977 0000-0003-3032-2556 |
url |
http://dx.doi.org/10.3390/molecules17055255 http://hdl.handle.net/11449/26323 |
identifier_str_mv |
Molecules. Basel: Mdpi Ag, v. 17, n. 5, p. 5255-5268, 2012. 1420-3049 10.3390/molecules17055255 WOS:000304587600040 WOS000304587600040.pdf 7927877224326837 3538253640602977 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 3.098 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5255-5268 application/pdf |
dc.publisher.none.fl_str_mv |
Mdpi Ag |
publisher.none.fl_str_mv |
Mdpi Ag |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965205300510720 |