Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro

Detalhes bibliográficos
Autor(a) principal: Pedroso,Rita Cassia N.
Data de Publicação: 2017
Outros Autores: Branquinho,Núbia Angelica A., Hara,Alessandra C.B.A.M., Costa,Alan Carlos, Silva,Fabiano Guimarães, Pimenta,Leticia P., Silva,Marcio Luiz A., Cunha,Wilson Roberto, Pauletti,Patricia M., Januario,Ana Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466
Resumo: ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.
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spelling Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitroFlavonoidHyptis marrubioidesIn vitro cultureLight qualityABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.Sociedade Brasileira de Farmacognosia2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466Revista Brasileira de Farmacognosia v.27 n.4 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2016.12.004info:eu-repo/semantics/openAccessPedroso,Rita Cassia N.Branquinho,Núbia Angelica A.Hara,Alessandra C.B.A.M.Costa,Alan CarlosSilva,Fabiano GuimarãesPimenta,Leticia P.Silva,Marcio Luiz A.Cunha,Wilson RobertoPauletti,Patricia M.Januario,Ana Helenaeng2017-08-31T00:00:00Zoai:scielo:S0102-695X2017000400466Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2017-08-31T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
title Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
spellingShingle Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
Pedroso,Rita Cassia N.
Flavonoid
Hyptis marrubioides
In vitro culture
Light quality
title_short Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
title_full Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
title_fullStr Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
title_full_unstemmed Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
title_sort Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
author Pedroso,Rita Cassia N.
author_facet Pedroso,Rita Cassia N.
Branquinho,Núbia Angelica A.
Hara,Alessandra C.B.A.M.
Costa,Alan Carlos
Silva,Fabiano Guimarães
Pimenta,Leticia P.
Silva,Marcio Luiz A.
Cunha,Wilson Roberto
Pauletti,Patricia M.
Januario,Ana Helena
author_role author
author2 Branquinho,Núbia Angelica A.
Hara,Alessandra C.B.A.M.
Costa,Alan Carlos
Silva,Fabiano Guimarães
Pimenta,Leticia P.
Silva,Marcio Luiz A.
Cunha,Wilson Roberto
Pauletti,Patricia M.
Januario,Ana Helena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pedroso,Rita Cassia N.
Branquinho,Núbia Angelica A.
Hara,Alessandra C.B.A.M.
Costa,Alan Carlos
Silva,Fabiano Guimarães
Pimenta,Leticia P.
Silva,Marcio Luiz A.
Cunha,Wilson Roberto
Pauletti,Patricia M.
Januario,Ana Helena
dc.subject.por.fl_str_mv Flavonoid
Hyptis marrubioides
In vitro culture
Light quality
topic Flavonoid
Hyptis marrubioides
In vitro culture
Light quality
description ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2016.12.004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.27 n.4 2017
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
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repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
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