Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000300011 |
Resumo: | Monoterpenes have been identified as responsible of important therapeutic effects of plant-extracts. In this work, we try to compare the cytotoxic effect of six monoterpenes (carvacrol, thymol, carveol, carvone, eugenol and isopulegol) as well as their molecular mechanisms. The in vitro antitumor activity of the tested products, evaluated against five tumor cell lines, show that the carvacrol is the most cytotoxic monoterpene. The investigation of an eventual synergistic effect of the six natural monoterpenes with two anticancer drugs revealed that there is a significant synergy between them (p<5%). On the other hand, the effect of the tested products on cell cycle progression was examined by flow cytometry after DNA staining in order to investigate the molecular mechanism of their cytotoxic activity. The results revealed that carvacrol and carveol stopped the cell cycle progression in S phase; however, thymol and isopulegol stopped it in G0/G1 phase. Regarding carvone and eugenol, no effect on cell cycle was observed. |
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Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysisAntitumor activitycarvacrolcell cyclemonoterpenessynergyMonoterpenes have been identified as responsible of important therapeutic effects of plant-extracts. In this work, we try to compare the cytotoxic effect of six monoterpenes (carvacrol, thymol, carveol, carvone, eugenol and isopulegol) as well as their molecular mechanisms. The in vitro antitumor activity of the tested products, evaluated against five tumor cell lines, show that the carvacrol is the most cytotoxic monoterpene. The investigation of an eventual synergistic effect of the six natural monoterpenes with two anticancer drugs revealed that there is a significant synergy between them (p<5%). On the other hand, the effect of the tested products on cell cycle progression was examined by flow cytometry after DNA staining in order to investigate the molecular mechanism of their cytotoxic activity. The results revealed that carvacrol and carveol stopped the cell cycle progression in S phase; however, thymol and isopulegol stopped it in G0/G1 phase. Regarding carvone and eugenol, no effect on cell cycle was observed.Sociedade Brasileira de Farmacognosia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000300011Revista Brasileira de Farmacognosia v.22 n.3 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2012005000021info:eu-repo/semantics/openAccessJaafari,AbdeslamTilaoui,MounirMouse,Hassan AitM'bark,Lahcen AitAboufatima,RachidaChait,AbderrahmaneLepoivre,MichelZyad,Abdelmajideng2012-05-09T00:00:00Zoai:scielo:S0102-695X2012000300011Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-05-09T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
title |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
spellingShingle |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis Jaafari,Abdeslam Antitumor activity carvacrol cell cycle monoterpenes synergy |
title_short |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
title_full |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
title_fullStr |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
title_full_unstemmed |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
title_sort |
Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis |
author |
Jaafari,Abdeslam |
author_facet |
Jaafari,Abdeslam Tilaoui,Mounir Mouse,Hassan Ait M'bark,Lahcen Ait Aboufatima,Rachida Chait,Abderrahmane Lepoivre,Michel Zyad,Abdelmajid |
author_role |
author |
author2 |
Tilaoui,Mounir Mouse,Hassan Ait M'bark,Lahcen Ait Aboufatima,Rachida Chait,Abderrahmane Lepoivre,Michel Zyad,Abdelmajid |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Jaafari,Abdeslam Tilaoui,Mounir Mouse,Hassan Ait M'bark,Lahcen Ait Aboufatima,Rachida Chait,Abderrahmane Lepoivre,Michel Zyad,Abdelmajid |
dc.subject.por.fl_str_mv |
Antitumor activity carvacrol cell cycle monoterpenes synergy |
topic |
Antitumor activity carvacrol cell cycle monoterpenes synergy |
description |
Monoterpenes have been identified as responsible of important therapeutic effects of plant-extracts. In this work, we try to compare the cytotoxic effect of six monoterpenes (carvacrol, thymol, carveol, carvone, eugenol and isopulegol) as well as their molecular mechanisms. The in vitro antitumor activity of the tested products, evaluated against five tumor cell lines, show that the carvacrol is the most cytotoxic monoterpene. The investigation of an eventual synergistic effect of the six natural monoterpenes with two anticancer drugs revealed that there is a significant synergy between them (p<5%). On the other hand, the effect of the tested products on cell cycle progression was examined by flow cytometry after DNA staining in order to investigate the molecular mechanism of their cytotoxic activity. The results revealed that carvacrol and carveol stopped the cell cycle progression in S phase; however, thymol and isopulegol stopped it in G0/G1 phase. Regarding carvone and eugenol, no effect on cell cycle was observed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000300011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000300011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2012005000021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.22 n.3 2012 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122467441180672 |