Antiviral activity of fractions from leaves of Piper regnelli var. pallescens

Detalhes bibliográficos
Autor(a) principal: Bertol,Jéssica W.
Data de Publicação: 2012
Outros Autores: Santos,Patricia R., Rodrigues,Juliana, Cortez,Diógenes A. G., D. Filho,Benedito P., Nakamura,Celso V., Ueda-Nakamura,Tania
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000600012
Resumo: This study investigated the antiviral potential of fractions and eupomatenoid-5 obtained from Piper regnelli (Miq.) C. DC., Piperaceae, leaves against bovine herpesvirus-1 (BHV-1) and poliovirus. VERO cell monolayers in 96-well cell culture plates were infected with BHV-1 or poliovirus and incubated in the presence and absence of samples for 48 h. The cells were then fixed and stained with sulforhodamine B, and the virus-induced cytopathic effect was measured in a 96-well plate reader at 530 nm. Cytotoxicity was assessed by incubating the cell monolayers with samples for 72 h. The hexane, chloroform, chloroform/ethyl acetate (19:1), and chloroform/ethyl acetate (9:1) fractions showed activity against BHV-1. The chloroform, chloroform/ethyl acetate (19:1), chloroform/ethyl acetate (9:1), chloroform/ethyl acetate (1:1), and ethyl acetate fractions were active against poliovirus. The chloroform/ethyl acetate (9:1) fraction presented the best selectivity index for both viruses. The present study reports the antiviral activity of the extract and fractions of P. regnelli leaves.
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spelling Antiviral activity of fractions from leaves of Piper regnelli var. pallescensantiviralbovine herpesvirusPiper regnellipoliovirusThis study investigated the antiviral potential of fractions and eupomatenoid-5 obtained from Piper regnelli (Miq.) C. DC., Piperaceae, leaves against bovine herpesvirus-1 (BHV-1) and poliovirus. VERO cell monolayers in 96-well cell culture plates were infected with BHV-1 or poliovirus and incubated in the presence and absence of samples for 48 h. The cells were then fixed and stained with sulforhodamine B, and the virus-induced cytopathic effect was measured in a 96-well plate reader at 530 nm. Cytotoxicity was assessed by incubating the cell monolayers with samples for 72 h. The hexane, chloroform, chloroform/ethyl acetate (19:1), and chloroform/ethyl acetate (9:1) fractions showed activity against BHV-1. The chloroform, chloroform/ethyl acetate (19:1), chloroform/ethyl acetate (9:1), chloroform/ethyl acetate (1:1), and ethyl acetate fractions were active against poliovirus. The chloroform/ethyl acetate (9:1) fraction presented the best selectivity index for both viruses. The present study reports the antiviral activity of the extract and fractions of P. regnelli leaves.Sociedade Brasileira de Farmacognosia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000600012Revista Brasileira de Farmacognosia v.22 n.6 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2012005000110info:eu-repo/semantics/openAccessBertol,Jéssica W.Santos,Patricia R.Rodrigues,JulianaCortez,Diógenes A. G.D. Filho,Benedito P.Nakamura,Celso V.Ueda-Nakamura,Taniaeng2012-12-06T00:00:00Zoai:scielo:S0102-695X2012000600012Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-12-06T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
title Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
spellingShingle Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
Bertol,Jéssica W.
antiviral
bovine herpesvirus
Piper regnelli
poliovirus
title_short Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
title_full Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
title_fullStr Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
title_full_unstemmed Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
title_sort Antiviral activity of fractions from leaves of Piper regnelli var. pallescens
author Bertol,Jéssica W.
author_facet Bertol,Jéssica W.
Santos,Patricia R.
Rodrigues,Juliana
Cortez,Diógenes A. G.
D. Filho,Benedito P.
Nakamura,Celso V.
Ueda-Nakamura,Tania
author_role author
author2 Santos,Patricia R.
Rodrigues,Juliana
Cortez,Diógenes A. G.
D. Filho,Benedito P.
Nakamura,Celso V.
Ueda-Nakamura,Tania
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bertol,Jéssica W.
Santos,Patricia R.
Rodrigues,Juliana
Cortez,Diógenes A. G.
D. Filho,Benedito P.
Nakamura,Celso V.
Ueda-Nakamura,Tania
dc.subject.por.fl_str_mv antiviral
bovine herpesvirus
Piper regnelli
poliovirus
topic antiviral
bovine herpesvirus
Piper regnelli
poliovirus
description This study investigated the antiviral potential of fractions and eupomatenoid-5 obtained from Piper regnelli (Miq.) C. DC., Piperaceae, leaves against bovine herpesvirus-1 (BHV-1) and poliovirus. VERO cell monolayers in 96-well cell culture plates were infected with BHV-1 or poliovirus and incubated in the presence and absence of samples for 48 h. The cells were then fixed and stained with sulforhodamine B, and the virus-induced cytopathic effect was measured in a 96-well plate reader at 530 nm. Cytotoxicity was assessed by incubating the cell monolayers with samples for 72 h. The hexane, chloroform, chloroform/ethyl acetate (19:1), and chloroform/ethyl acetate (9:1) fractions showed activity against BHV-1. The chloroform, chloroform/ethyl acetate (19:1), chloroform/ethyl acetate (9:1), chloroform/ethyl acetate (1:1), and ethyl acetate fractions were active against poliovirus. The chloroform/ethyl acetate (9:1) fraction presented the best selectivity index for both viruses. The present study reports the antiviral activity of the extract and fractions of P. regnelli leaves.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000600012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000600012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-695X2012005000110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.22 n.6 2012
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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