Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies

Detalhes bibliográficos
Autor(a) principal: Zhou,Qiang
Data de Publicação: 2018
Outros Autores: Yan,He, Li,Rui, Li,Xingliang, Wei,Jingyan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000400451
Resumo: Abstract A selective and sensitive liquid chromatography tandem with mass spectrometry was developed and validated for accurate determination of monotropein in rat plasma and tissues. All biological samples were prepared by simple protein precipitation method using catalpol as an internal standard. The analyte and internal standard were separated on a C18 analytical column with 2 min of run time, at flow rate of 0.5 ml/min. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with negative-ion electrospray ionization by selected-reaction monitoring of the transitions at m/z 389→147 for monotropein and m/z 361→169 for the internal standard. The calibration curves for plasma and tissue samples were linear over the concentration range of 4–2000 ng/ml, with a lower limit of quantification of 4 ng/ml. The method was successfully applied to a pharmacokinetic and tissue distribution study of monotropein in rats.
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spelling Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studiesMonotropeinPharmacokineticsTissue distributionLC–MS/MSAbstract A selective and sensitive liquid chromatography tandem with mass spectrometry was developed and validated for accurate determination of monotropein in rat plasma and tissues. All biological samples were prepared by simple protein precipitation method using catalpol as an internal standard. The analyte and internal standard were separated on a C18 analytical column with 2 min of run time, at flow rate of 0.5 ml/min. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with negative-ion electrospray ionization by selected-reaction monitoring of the transitions at m/z 389→147 for monotropein and m/z 361→169 for the internal standard. The calibration curves for plasma and tissue samples were linear over the concentration range of 4–2000 ng/ml, with a lower limit of quantification of 4 ng/ml. The method was successfully applied to a pharmacokinetic and tissue distribution study of monotropein in rats.Sociedade Brasileira de Farmacognosia2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000400451Revista Brasileira de Farmacognosia v.28 n.4 2018reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2018.05.005info:eu-repo/semantics/openAccessZhou,QiangYan,HeLi,RuiLi,XingliangWei,Jingyaneng2018-08-13T00:00:00Zoai:scielo:S0102-695X2018000400451Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2018-08-13T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
title Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
spellingShingle Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
Zhou,Qiang
Monotropein
Pharmacokinetics
Tissue distribution
LC–MS/MS
title_short Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
title_full Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
title_fullStr Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
title_full_unstemmed Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
title_sort Quantitative determination of monotropein in rat plasma and tissue by LC–MS/MS and its application to pharmacokinetic and tissue distribution studies
author Zhou,Qiang
author_facet Zhou,Qiang
Yan,He
Li,Rui
Li,Xingliang
Wei,Jingyan
author_role author
author2 Yan,He
Li,Rui
Li,Xingliang
Wei,Jingyan
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zhou,Qiang
Yan,He
Li,Rui
Li,Xingliang
Wei,Jingyan
dc.subject.por.fl_str_mv Monotropein
Pharmacokinetics
Tissue distribution
LC–MS/MS
topic Monotropein
Pharmacokinetics
Tissue distribution
LC–MS/MS
description Abstract A selective and sensitive liquid chromatography tandem with mass spectrometry was developed and validated for accurate determination of monotropein in rat plasma and tissues. All biological samples were prepared by simple protein precipitation method using catalpol as an internal standard. The analyte and internal standard were separated on a C18 analytical column with 2 min of run time, at flow rate of 0.5 ml/min. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with negative-ion electrospray ionization by selected-reaction monitoring of the transitions at m/z 389→147 for monotropein and m/z 361→169 for the internal standard. The calibration curves for plasma and tissue samples were linear over the concentration range of 4–2000 ng/ml, with a lower limit of quantification of 4 ng/ml. The method was successfully applied to a pharmacokinetic and tissue distribution study of monotropein in rats.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000400451
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2018000400451
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjp.2018.05.005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.28 n.4 2018
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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