Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti

Detalhes bibliográficos
Autor(a) principal: Martin,Paula Garcia
Data de Publicação: 2018
Outros Autores: Tavares,Juliana Sala de Faria, Mangolin,Claudete Aparecida, Machado,Maria de Fátima Pires da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ornamental Horticulture (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400415
Resumo: ABSTRACT Somaclones of mandacaru cactus (Cereus peruvianus Mill.) with typical and atypical shoots were regenerated from a callus. Since the genetic relationship among regenerated somaclones has not been investigated, current study employed heterologous microsatellite primers to examine the molecular diversity within and among somaclones, showing typical and atypical phenotypes with high morphological divergence. Nei identity value calculated between the somaclones was high (I = 0.929) and AMOVA showed higher genetic variation within (96%) than among (4%) the samples of somaclones. The polymorphism in the microsatellite loci indicated high levels of mean observed and expected heterozygosity in atypical somaclones, presumably with high adaptive potential and as source of genotypes for generation of new varieties of ornamental cacti. On the other hand, low molecular divergence between typical and atypical morphologies of somaclones is a promising perspective for use of the atypical somaclones as source of chemical compounds of commercial and industrial interest. The somaclonal variations occurring in vitro callus culture has generated phenotypically differentiated subpopulations with low molecular divergence, however with high genetic variability, enough to be recommended as a source of genotypes to generate new varieties of ornamental cacti and of plants with new traits, necessary for breeding programs.
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spelling Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cactimicropropagationcactusCactaceaemicrosatellite transferabilitygenetic diversityABSTRACT Somaclones of mandacaru cactus (Cereus peruvianus Mill.) with typical and atypical shoots were regenerated from a callus. Since the genetic relationship among regenerated somaclones has not been investigated, current study employed heterologous microsatellite primers to examine the molecular diversity within and among somaclones, showing typical and atypical phenotypes with high morphological divergence. Nei identity value calculated between the somaclones was high (I = 0.929) and AMOVA showed higher genetic variation within (96%) than among (4%) the samples of somaclones. The polymorphism in the microsatellite loci indicated high levels of mean observed and expected heterozygosity in atypical somaclones, presumably with high adaptive potential and as source of genotypes for generation of new varieties of ornamental cacti. On the other hand, low molecular divergence between typical and atypical morphologies of somaclones is a promising perspective for use of the atypical somaclones as source of chemical compounds of commercial and industrial interest. The somaclonal variations occurring in vitro callus culture has generated phenotypically differentiated subpopulations with low molecular divergence, however with high genetic variability, enough to be recommended as a source of genotypes to generate new varieties of ornamental cacti and of plants with new traits, necessary for breeding programs.Sociedade Brasileira de Floricultura e Plantas Ornamentais2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400415Ornamental Horticulture v.24 n.4 2018reponame:Ornamental Horticulture (Campinas)instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)instacron:SBFPO10.14295/oh.v24i4.1256info:eu-repo/semantics/openAccessMartin,Paula GarciaTavares,Juliana Sala de FariaMangolin,Claudete AparecidaMachado,Maria de Fátima Pires da Silvaeng2019-02-20T00:00:00Zoai:scielo:S2447-536X2018000400415Revistahttp://ornamentalhorticulture.emnuvens.com.br/rbho/indexhttps://old.scielo.br/oai/scielo-oai.php||revista.ornamentalhorticulture@gmail.com2447-536X2447-536Xopendoar:2019-02-20T00:00Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)false
dc.title.none.fl_str_mv Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
title Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
spellingShingle Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
Martin,Paula Garcia
micropropagation
cactus
Cactaceae
microsatellite transferability
genetic diversity
title_short Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
title_full Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
title_fullStr Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
title_full_unstemmed Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
title_sort Somaclones of mandacaru (Cactaceae) with high morphological divergence may generate new varieties of ornamental cacti
author Martin,Paula Garcia
author_facet Martin,Paula Garcia
Tavares,Juliana Sala de Faria
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
author_role author
author2 Tavares,Juliana Sala de Faria
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Martin,Paula Garcia
Tavares,Juliana Sala de Faria
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
dc.subject.por.fl_str_mv micropropagation
cactus
Cactaceae
microsatellite transferability
genetic diversity
topic micropropagation
cactus
Cactaceae
microsatellite transferability
genetic diversity
description ABSTRACT Somaclones of mandacaru cactus (Cereus peruvianus Mill.) with typical and atypical shoots were regenerated from a callus. Since the genetic relationship among regenerated somaclones has not been investigated, current study employed heterologous microsatellite primers to examine the molecular diversity within and among somaclones, showing typical and atypical phenotypes with high morphological divergence. Nei identity value calculated between the somaclones was high (I = 0.929) and AMOVA showed higher genetic variation within (96%) than among (4%) the samples of somaclones. The polymorphism in the microsatellite loci indicated high levels of mean observed and expected heterozygosity in atypical somaclones, presumably with high adaptive potential and as source of genotypes for generation of new varieties of ornamental cacti. On the other hand, low molecular divergence between typical and atypical morphologies of somaclones is a promising perspective for use of the atypical somaclones as source of chemical compounds of commercial and industrial interest. The somaclonal variations occurring in vitro callus culture has generated phenotypically differentiated subpopulations with low molecular divergence, however with high genetic variability, enough to be recommended as a source of genotypes to generate new varieties of ornamental cacti and of plants with new traits, necessary for breeding programs.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400415
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2018000400415
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.14295/oh.v24i4.1256
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais
publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais
dc.source.none.fl_str_mv Ornamental Horticulture v.24 n.4 2018
reponame:Ornamental Horticulture (Campinas)
instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)
instacron:SBFPO
instname_str Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)
instacron_str SBFPO
institution SBFPO
reponame_str Ornamental Horticulture (Campinas)
collection Ornamental Horticulture (Campinas)
repository.name.fl_str_mv Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)
repository.mail.fl_str_mv ||revista.ornamentalhorticulture@gmail.com
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