Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

Detalhes bibliográficos
Autor(a) principal: Bombonato,Juliana Rodrigues
Data de Publicação: 2019
Outros Autores: Bonatelli,Isabel Aparecida Silva, Silva,Gislaine Angélica Rodrigues, Moraes,Evandro Marsola, Zappi,Daniela Cristina, Taylor,Nigel P., Franco,Fernando Faria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
Resumo: Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.
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spelling Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)CactaceaeCereuscross-generaSSR markersTransferabilityAbstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.Sociedade Brasileira de Genética2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087Genetics and Molecular Biology v.42 n.1 2019reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2017-0293info:eu-repo/semantics/openAccessBombonato,Juliana RodriguesBonatelli,Isabel Aparecida SilvaSilva,Gislaine Angélica RodriguesMoraes,Evandro MarsolaZappi,Daniela CristinaTaylor,Nigel P.Franco,Fernando Fariaeng2019-03-15T00:00:00Zoai:scielo:S1415-47572019000100087Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2019-03-15T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
title Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
spellingShingle Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
Bombonato,Juliana Rodrigues
Cactaceae
Cereus
cross-genera
SSR markers
Transferability
title_short Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
title_full Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
title_fullStr Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
title_full_unstemmed Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
title_sort Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
author Bombonato,Juliana Rodrigues
author_facet Bombonato,Juliana Rodrigues
Bonatelli,Isabel Aparecida Silva
Silva,Gislaine Angélica Rodrigues
Moraes,Evandro Marsola
Zappi,Daniela Cristina
Taylor,Nigel P.
Franco,Fernando Faria
author_role author
author2 Bonatelli,Isabel Aparecida Silva
Silva,Gislaine Angélica Rodrigues
Moraes,Evandro Marsola
Zappi,Daniela Cristina
Taylor,Nigel P.
Franco,Fernando Faria
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bombonato,Juliana Rodrigues
Bonatelli,Isabel Aparecida Silva
Silva,Gislaine Angélica Rodrigues
Moraes,Evandro Marsola
Zappi,Daniela Cristina
Taylor,Nigel P.
Franco,Fernando Faria
dc.subject.por.fl_str_mv Cactaceae
Cereus
cross-genera
SSR markers
Transferability
topic Cactaceae
Cereus
cross-genera
SSR markers
Transferability
description Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2017-0293
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.42 n.1 2019
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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